Covalent binding and fluorimetric determination of dialdehydes using aminated silica nanoparticles and ethylenediamine fluorescein
- Authors: Filenko I.A.1, Golodukhina S.V.1, Usol’tseva L.O.1, Adamova E.M.1, Beklemishev M.K.1
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Affiliations:
- Department of Chemistry
- Issue: Vol 72, No 9 (2017)
- Pages: 977-985
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182622
- DOI: https://doi.org/10.1134/S1061934817090040
- ID: 182622
Cite item
Abstract
The possibility of formation of a ternary (sandwich) compound of a dialdehyde (malondialdehyde, glutaraldehyde, or glyoxal) with ethylenediamine fluorescein thiocarbamyl (EDF) and silica nanoparticles noncovalently modified with polyethyleneimine (SiO2/PEI) with the subsequent fluorimetric determination of the dialdehyde was demonstrated. The mixed Schiff base SiO2/PEI–dialdehyde–EDF (sandwich) is formed in an acetic acid solution on heating in a water bath. The sandwich and the excess of SiO2/PEI were separated from the unreacted fluorophore by centrifugation; the precipitate was washed and resuspended in water, and the fluorescence of solution was measured (λex = 470 nm, λem = 520 nm). The duration of an analytical cycle was no longer than a half-hour. The limit of detection of dialdehydes in pure water is 1 × 10–5 M, and the analytical concentration range is 2 × 10–5–3 × 10–4 M (for malondialdehyde). The repeatability RSDs in this concentration range were 3–5% (n = 3). The comparable concentrations of sulfamethoxazole, sulfadiazine, pyracetam, and chloramphenicol and 1 × 10–5 M of ceftriaxone, ceftazidime, metamizole (analgin), isoniazid, and amikacin caused no interference with the determination of 3 × 10–4 M malondialdehyde; protein noticeably interfered. The determination of glyoxal and glutaraldehyde in disinfectants was carried out.
About the authors
I. A. Filenko
Department of Chemistry
Email: mkb@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
S. V. Golodukhina
Department of Chemistry
Email: mkb@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
L. O. Usol’tseva
Department of Chemistry
Email: mkb@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
E. M. Adamova
Department of Chemistry
Email: mkb@analyt.chem.msu.ru
Russian Federation, Moscow, 119991
M. K. Beklemishev
Department of Chemistry
Author for correspondence.
Email: mkb@analyt.chem.msu.ru
Russian Federation, Moscow, 119991