Quantitative determination of diterpene acids in garden sage leaves
- Authors: Khaliullina A.S.1, Khaziev R.S.1, Salamatin A.A.2
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Affiliations:
- Pharmaceutical Faculty
- Lobachevsky Institute of Mathematics and Mechanics
- Issue: Vol 72, No 7 (2017)
- Pages: 810-814
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182575
- DOI: https://doi.org/10.1134/S1061934817070073
- ID: 182575
Cite item
Abstract
A procedure is developed for the quantitative determination of diterpene acids in garden sage leaves by UV spectrophotometry at the wavelength 285 nm. The target group of compounds was selectively extracted by petroleum ether 40/70. It was shown that the completeness of extraction is determined mainly by the number of portions of the pure solvent: at the optimum ratio of the mass of the weighed portion to the volume of solvent 1 g/200 mL, double extraction is sufficient. The duration of each extraction is 20 min. The procedure was used in the analysis of samples of garden sage leaves from various producers. It was found that the concentration of diterpene acids in samples varied from 2.1 to 3.6 wt % (in terms of carnosic acid). The error of a single determination of the sum of diterpene acids in garden sage leaves is ±2.38% (P = 0.95).
About the authors
A. S. Khaliullina
Pharmaceutical Faculty
Author for correspondence.
Email: anela_90@mail.ru
Russian Federation, Kazan, 420012
R. Sh. Khaziev
Pharmaceutical Faculty
Email: anela_90@mail.ru
Russian Federation, Kazan, 420012
A. A. Salamatin
Lobachevsky Institute of Mathematics and Mechanics
Email: anela_90@mail.ru
Russian Federation, Kazan, 420008