A luminescence immunoassay test method for determining benzo[a]pyrene in natural water
- Authors: Beloglazova N.V.1, Shmelin P.S.1,2, Novikova A.S.1, Goryacheva I.Y.1
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Affiliations:
- Chernyshevskii Saratov State University
- Tekhnomash
- Issue: Vol 72, No 6 (2017)
- Pages: 597-601
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182475
- DOI: https://doi.org/10.1134/S106193481706003X
- ID: 182475
Cite item
Abstract
A test method is developed for determining benzo[a]pyrene in natural water, based on the use of a polyethylene filter (frit) with adsorbed specific antibodies, placed within a transparent column. In passing a test solution, the analyte is adsorbed on the frit similarly to the process implemented in immunoaffinity preconcentration. The added conjugate of a labeled analyte takes the remained vacant binding sites of antibodies. Luminescent semiconductor nanoparticles (quantum dots) CdSe/ZnS, used as labels, enable visual determination under irradiation with UV light. The limit of detection for benzo[a]pyrene in water is ~0.5 ng/mL.
About the authors
N. V. Beloglazova
Chernyshevskii Saratov State University
Email: goryachevaiy@mail.ru
Russian Federation, Saratov, 410012
P. S. Shmelin
Chernyshevskii Saratov State University; Tekhnomash
Email: goryachevaiy@mail.ru
Russian Federation, Saratov, 410012; Moscow, 121108
A. S. Novikova
Chernyshevskii Saratov State University
Email: goryachevaiy@mail.ru
Russian Federation, Saratov, 410012
I. Yu. Goryacheva
Chernyshevskii Saratov State University
Author for correspondence.
Email: goryachevaiy@mail.ru
Russian Federation, Saratov, 410012