Headspace gas-chromatographic determination of formaldehyde in urine
- Authors: Alekseenko A.N.1, Zhurba O.M.1,2, Efimova N.V.1,2, Rukavishnikov V.S.1,2
-
Affiliations:
- East Siberian Institute of Medical and Environmental Research
- Irkutsk Scientific Center, Siberian Branch
- Issue: Vol 72, No 1 (2017)
- Pages: 83-86
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182268
- DOI: https://doi.org/10.1134/S1061934816110022
- ID: 182268
Cite item
Abstract
A method is proposed for determining formaldehyde by headspace gas chromatography using an o-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBHA) derivatizing agent. Formaldehyde in urine was derivatized to o-(pentafluorobenzyl)oxime and extracted by heating an urine sample with PFBHA in a sealed vial. Gas-chromatographic analysis of the headspace was performed in the temperature-programmed mode using an HP-5 capillary column with a flame ionization detector. The limit of detection is 3.5 μg/L. The accuracy of the procedure, equivalent to an extended relative uncertainty, does not exceed 21%. The examination of the procedure using urine samples of the population of the Irkutsk region has shown that the regional reference levels of formaldehyde concentration are in the range 44–83 μg/L.
About the authors
A. N. Alekseenko
East Siberian Institute of Medical and Environmental Research
Author for correspondence.
Email: labchem99@gmail.com
Russian Federation, Angarsk, 665827
O. M. Zhurba
East Siberian Institute of Medical and Environmental Research; Irkutsk Scientific Center, Siberian Branch
Email: labchem99@gmail.com
Russian Federation, Angarsk, 665827; Irkutsk, 664033
N. V. Efimova
East Siberian Institute of Medical and Environmental Research; Irkutsk Scientific Center, Siberian Branch
Email: labchem99@gmail.com
Russian Federation, Angarsk, 665827; Irkutsk, 664033
V. S. Rukavishnikov
East Siberian Institute of Medical and Environmental Research; Irkutsk Scientific Center, Siberian Branch
Email: labchem99@gmail.com
Russian Federation, Angarsk, 665827; Irkutsk, 664033