Determination of 2,4-dichlorophenoxyacetic acid in cereals by fluorescence polarization immunoassay
- Authors: Boroduleva A.Y.1, Eremin S.A.1
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Affiliations:
- Department of Chemistry
- Issue: Vol 71, No 9 (2016)
- Pages: 949-954
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182082
- DOI: https://doi.org/10.1134/S1061934816090045
- ID: 182082
Cite item
Abstract
A fluorescence polarization immunoassay (FPIA) procedure is developed for the determination of 2,4-dichlorophenoxyacetic acid in cereal grains using a Sentry-200 portable device. We synthesized tracers, that is, antigens labeled with fluorescein derivatives, based on two fluorescent compounds and two chlorophenoxyacids. The tracer synthesized from 2,4-dichlorophenoxyacetic acid and 4-aminomethylfluorescein is found to be optimal for the FPIA of 2,4-dichlorophenoxyacetic acid. The limit of detection for 2,4-dichlorophenoxyacetic acid in flour was 40 ng/g; the analytical range is from 80 to 1000 ng/g. The percentage of recovery was 85 ± 7% at the relative standard deviation (RSD) 2–10%.
About the authors
A. Yu. Boroduleva
Department of Chemistry
Author for correspondence.
Email: boroduleva.anna@gmail.com
Russian Federation, Moscow, 119991
S. A. Eremin
Department of Chemistry
Email: boroduleva.anna@gmail.com
Russian Federation, Moscow, 119991