Email this article Determination of protein adducts of organophosphorus nerve agents in blood plasma
- Authors: Koryagina N.L.1, Savel’eva E.I.1, Karakashev G.V.1, Babakov V.N.1, Dubrovskii Y.A.1, Ukolova E.S.1, Khlebnikova N.S.1, Murashko E.A.1, Koneva V.Y.1, Ukolov A.I.1, Kopeikin V.A.1, Radilov A.S.1
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Affiliations:
- Research Institute of Hygiene, Occupational Pathology, and Human Ecology
- Issue: Vol 71, No 8 (2016)
- Pages: 849-859
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182015
- DOI: https://doi.org/10.1134/S1061934816080086
- ID: 182015
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Abstract
A comparative study of the efficiency of procedures for the determination of the biomarkers of organophosphorus agents (OPAs) in blood plasma was performed. It was found that the gas chromatography–mass spectrometry determination of OPAs reactivated from the composition of protein adducts is a rapid method for the detection of exposure to OPAs. The liquid chromatography–mass spectrometry determination of phosphonylated butyrylcholinesterase and albumin fragments modified with OPA residues provides an opportunity to perform more sensitive and retrospective analysis. The tyrosine adducts of OPAs with serum albumin and other blood plasma proteins are not prone to dealkylation in the course of aging; in the series of the test markers, they possess the greatest diagnostic value because they make it possible to determine the precise structure a toxic agent after the longest time interval after exposure. The tentative limit of detection of OPA markers varies from 0.1 to 1.0 ng/mL.
About the authors
N. L. Koryagina
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Author for correspondence.
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
E. I. Savel’eva
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
G. V. Karakashev
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
V. N. Babakov
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
Ya. A. Dubrovskii
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
E. S. Ukolova
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
N. S. Khlebnikova
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
E. A. Murashko
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
V. Yu. Koneva
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
A. I. Ukolov
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
V. A. Kopeikin
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
A. S. Radilov
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
