Determination of a New Antiulcer Drug in Rat Blood Plasma by Liquid Chromatography–Mass Spectrometry
- Authors: Leonov K.A.1, Vishenkova D.A.2, Bykov V.V.3, Bakibaev A.A.4
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Affiliations:
- LLC Innovative Pharmacology Research
- National Research Tomsk Polytechnic University
- Siberian State Medical University
- National Research Tomsk State University
- Issue: Vol 74, No 14 (2019)
- Pages: 1447-1453
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/183398
- DOI: https://doi.org/10.1134/S1061934819140089
- ID: 183398
Cite item
Abstract
Synthesis of an innovative drug 9-(2,5-dihydroxyphenyl)-2-(4-ethoxyphenyl)-2,3,7,8-tetrahydro-1H-pyrido[1,2-a]pyrazine-1,4(6H)-dione (PPI) for the treatment of peptic ulcer with a new mechanism of action has necessitated the pharmacokinetics study of the substance in the blood plasma and investigation of its absorption and elimination processes. This paper describes the development and validation of a technique for the quantitative determination of new compound PPI in rat blood plasma by HPLC/MS. The method of solid-phase extraction is applied to extract PPI from blood plasma in the process of biological sample preparation. The lower limit of quantification is 0.1 ng/mL with a linearity range from 0.1 to 1000 ng/mL. By the developed technique, the sample analysis of rat blood plasma was carried out after the introduction of PPI pharmaceutical substance in a dose of 20 mg/kg, a pharmacokinetic profile of the substance was established, and pharmacokinetic parameters were calculated in order to judge the degrees and rates of absorption and excretion of the new antiulcer drug in the blood of laboratory rats.
About the authors
K. A. Leonov
LLC Innovative Pharmacology Research
Author for correspondence.
Email: leonov_k90@mail.ru
Russian Federation, Tomsk, 634021
D. A. Vishenkova
National Research Tomsk Polytechnic University
Email: leonov_k90@mail.ru
Russian Federation, Tomsk, 634034
V. V. Bykov
Siberian State Medical University
Email: leonov_k90@mail.ru
Russian Federation, Tomsk, 634055
A. A. Bakibaev
National Research Tomsk State University
Email: leonov_k90@mail.ru
Russian Federation, Tomsk, 634050
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