Determination of triacylglycerols of manketti oil by reversed-phase HPLC

Abstract

Using reversed-phase HPLC with refractometric detection, we determined the composition of triacylglycerols (TAGs) of manketti oil and calculated its fatty acid composition. It was shown that the oil consists of octadecatrienoic (34.6 ± 0.8 mol %), linoleic (35.4 ± 0.8 mol %), oleic (14.6 ± 0.4 mol %), palmitic (7.2 ± 0.3 mol %), and stearic acids. Using spectrophotometric detection, for the oils of centrathus and jacaranda seeds, it was demonstrated that octadecatrienoic acid is presented mainly by α-eleostearic acid and small impurities of β-eleostearic (2.8 mol %) and jacarandic (0.3 mol %) acids. The results of the quantitative determination of TAGs, obtained by refractometric detection (with corrections of the peak areas to the calculated values of the refractive indices of TAGs), are consistent with the data obtained using spectrophotometric detection.