Voltammetric determination of cholesterol in human blood serum

Abstract

Cholesterol has a number of important functions in a human body. The disorders of cholesterol biosynthesis increase the risk of the development of cardiovascular diseases and worsens the function of the immune system. This study is devoted to the development of a method for determining the concentration of cholesterol in blood serum using voltammetry. We selected the following working conditions for the determination of cholesterol: a phosphate buffer solution with pH 6.86 was a supporting electrolyte; potential sweep rate was W = 30 mV/s; and recording was carried out in the constant-current mode. An organomodified electrode was used as a working electrode. The peak was observed at +0.98 V. The dependence of the electrooxidation current of cholesterol on its concentration was linear in the range of 0.1–50 μM with a limit of detection of 0.01 μM. The results of the determination of cholesterol in blood serum by voltammetry were compared to those obtained by the fluorimetric method.