Optimization of a method for the determination of a mustard gas biomarker in human blood plasma by liquid chromatography–high-resolution mass spectrometry
- Authors: Braun A.V.1,2, Rybal’chenko I.V.1,2, Ponsov M.A.2, Stavitskaya Y.V.2, Tikhomirov L.A.1, Grechukhin A.P.1
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Affiliations:
- Kostroma State University
- Research Center 27 of the Ministry of Defense of the Russian Federation
- Issue: Vol 72, No 3 (2017)
- Pages: 303-308
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182343
- DOI: https://doi.org/10.1134/S1061934817030030
- ID: 182343
Cite item
Abstract
A method for the determination of a mustard gas biomarker (an S-hydroxyethylthioethyl adduct with albumin) in blood plasma was optimized with the use of HPLC with high-resolution tandem mass-spectrometric detection. This method is based on the hydrolysis of this adduct by the proteinase K enzyme with the formation of the following stable tripeptide with cysteine, proline, and phenylalanine: S-[HETE]-Cys- Pro-Phe. The conditions of the sample preparation of human plasma artificially contaminated by mustard gas (the selection of an aliquot portion volume and an enzyme for the hydrolysis), the mass-spectrometric detection (the selection of optimum pairs of ion reactions and high-resolution detection modes), and the gradient elution program in the HPLC separation of an analyzed mixture were optimized. The detection limit of mustard gas in human blood plasma was 1 ng/mL. The approach proposed was tested in the analysis of human blood plasma samples by the standard addition technique and also within the framework of the first official biomedical test carried out by the Organization for the Prohibition of Chemical Weapons (OPCW) in 2016, and it exhibited a good accuracy, reproducibility, and specificity of determination.
About the authors
A. V. Braun
Kostroma State University; Research Center 27 of the Ministry of Defense of the Russian Federation
Author for correspondence.
Email: avbraun@yandex.ru
Russian Federation, ul. Dzerzhinskogo 17, Kostroma, 156005; Brigadirskii per. 13, Moscow, 105005
I. V. Rybal’chenko
Kostroma State University; Research Center 27 of the Ministry of Defense of the Russian Federation
Email: avbraun@yandex.ru
Russian Federation, ul. Dzerzhinskogo 17, Kostroma, 156005; Brigadirskii per. 13, Moscow, 105005
M. A. Ponsov
Research Center 27 of the Ministry of Defense of the Russian Federation
Email: avbraun@yandex.ru
Russian Federation, Brigadirskii per. 13, Moscow, 105005
Ya. V. Stavitskaya
Research Center 27 of the Ministry of Defense of the Russian Federation
Email: avbraun@yandex.ru
Russian Federation, Brigadirskii per. 13, Moscow, 105005
L. A. Tikhomirov
Kostroma State University
Email: avbraun@yandex.ru
Russian Federation, ul. Dzerzhinskogo 17, Kostroma, 156005
A. P. Grechukhin
Kostroma State University
Email: avbraun@yandex.ru
Russian Federation, ul. Dzerzhinskogo 17, Kostroma, 156005