Chromatography–mass spectrometry determination of alkyl methylphosphonic acids in urine
- Authors: Koryagina N.L.1, Savel’eva E.I.1, Khlebnikova N.S.1, Ukolov A.I.1, Ukolova E.S.1, Karakashev G.V.1, Radilov A.S.1
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Affiliations:
- Research Institute of Hygiene, Occupational Pathology, and Human Ecology
- Issue: Vol 71, No 14 (2016)
- Pages: 1309-1318
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/182235
- DOI: https://doi.org/10.1134/S1061934816140069
- ID: 182235
Cite item
Abstract
The potentials of different chromatography–mass spectrometry methods for the determination of alkyl methylphosphonic acids (AMPAs)—the chemical markers of nerve agents in urine—are compared. The gas chromatography–mass spectrometry (GC–MS) characteristics of various volatile AMPA derivatives are studied. The preference of perfluorobenzyl derivatives over methyl, trimethylsilyl and tert-butyldimethylsilyl esters for the GC–MS determination of AMPAs in urine is demonstrated. An optimal technique for the determination of AMPAs in urine is HPLC combined with high-resolution MS2 mass spectrometry with the isotope–labeled forms of target compounds as internal standards. The detection limits of AMPAs in the proposed analytical procedures vary from 0.1 to 1.0 ng/mL.
About the authors
N. L. Koryagina
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Author for correspondence.
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
E. I. Savel’eva
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
N. S. Khlebnikova
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
A. I. Ukolov
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
E. S. Ukolova
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
G. V. Karakashev
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663
A. S. Radilov
Research Institute of Hygiene, Occupational Pathology, and Human Ecology
Email: koryagina@rihophe.ru
Russian Federation, pos. Kuz’molovskii, Vsevolozhskii region, Leningrad oblast, 188663