Optimization of solid phase microextraction procedure for determination of paraquat using reduction process
- Authors: Ghavidel F.1, Shahtaheri S.J.2, Torabbeigi M.3, Froushani A.R.4
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Affiliations:
- Department of Occupational Health Engineering, School of Public Health
- Department of Occupational Health Engineering, School of Public Health, Institute for Environmental Research
- Health, Safety and Environment School
- Department of Epidemiology and Biostatistics, School of Public Health
- Issue: Vol 71, No 7 (2016)
- Pages: 648-652
- Section: Articles
- URL: https://journals.rcsi.science/1061-9348/article/view/181842
- DOI: https://doi.org/10.1134/S106193481607008X
- ID: 181842
Cite item
Abstract
Headspace solid phase microextraction (HS-SPME) followed by gas chromatography–mass spectrometry was developed for the determination of paraquat in urine. The volatile product resulting from reduction of paraquat by sodium borohydride–nickel chloride was used for HS-SPME. The calibration curve was linear from 10–1000 ng/mL and the limit of detection was 0.1 ng/mL. The optimized methods were validated using 500 and 750 ng/mL concentrations of paraquat in the spiked urine samples. The recoveries obtained in this study were 98.2% for intra-day (n = 6) and 99.2% for inter-day (n = 6), respectively, with RSD lower than 1.1%.
About the authors
Fateme Ghavidel
Department of Occupational Health Engineering, School of Public Health
Email: shahtaheri@tums.ac.ir
Iran, Islamic Republic of, Tehran, 14155-6446
Seyed Jamaleddin Shahtaheri
Department of Occupational Health Engineering, School of Public Health, Institute for Environmental Research
Author for correspondence.
Email: shahtaheri@tums.ac.ir
Iran, Islamic Republic of, Tehran, 14155-6446
Marzieh Torabbeigi
Health, Safety and Environment School
Email: shahtaheri@tums.ac.ir
Iran, Islamic Republic of, Tehran
Abbas Rahimi Froushani
Department of Epidemiology and Biostatistics, School of Public Health
Email: shahtaheri@tums.ac.ir
Iran, Islamic Republic of, Tehran, 14155-6446