Determination of γ-hexachlorocyclohexane and its metabolites in biological samples from rat

Abstract

We have determined γ-hexachlorocyclohexane (lindane) and its metabolites in urine, serum and feces samples from rats using HPLC-UV-Vis and confirmation of mass with matrix assisted laser desorption/ ionization-time of flight (MALDI-TOF) analysis. Samples were collected from rats treated orally with lindane (17.6 mg/kg; 1/5 of LD₅₀) or vehicle for 2 weeks. Lindane and metabolites were extracted from samples with hexane and analyzed. The HPLC–MALDI-TOF is highly sensitive to the point of detecting very low level (5 ppm) of lindane and metabolites. The HPLC-UV-Vis analysis confirmed the presence of lindane in urine (386–1652 ppm), serum (207–371 ppm) and feces (5–74 ppm). Control samples had no peak corresponding to lindane. MALDI-TOF analysis of urine and serum samples showed a major peak at 293 m/z, whereas feces showed a minor peak at 292–293 m/z, which were consistent with the peak obtained for standard lindane (293 m/z). Our data indicates that HPLC-UV-Vis–MALDI-TOF combo method is sensitive for detecting and quantifying lindane and its metabolites in serum, urine and feces. Our results further showed that minor quantities of lindane and metabolites were excreted through feces confirming that the main pathway for excretion of lindane and metabolites is through urine.