INVESTIGATION OF BIOSPECIFIC INTERACTION BETWEEN DNA-APTAMER AND CARBON NANOPARTICLES

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Aptamers are DNA- and RNA-oligonucleotides that can selectively bind target molecules. Objective of our work was to investigate into interaction of biotinylated DNA-aptamer specific to human IgE with carbon nanoparticles covalently conjugated with streptavidin. Maximal level of specific interaction (about 40 pM per 1 mg of nanoparticles) was observed when concentration of aptamer was 100 nM.

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M. Rayev

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

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P. Khramtsov

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

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M. Bochkova

Institute of Ecology and Genetics of Microorganisms UB RAS

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V. Timganova

Institute of Ecology and Genetics of Microorganisms UB RAS

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S. Zamorina

Institute of Ecology and Genetics of Microorganisms UB RAS; Perm State National Research University

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M. Kropaneva

Perm State National Research University

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参考

  1. Zhou J., Rossi J. Aptamers as targeted therapeutics: Current potential and challenges. Nature Reviews Drug Discovery. 2017, 16 (Suppl.), 181-202.
  2. Posthuma-Trumpie G., Wichers J., Koets M., Ber-endsen L, van Amerongen A. Amorphous carbon nanoparticles: a versatile label for rapid diagnostic (immuno)assays. Analytical and Bioanalytical Chemistry. 2012, 402 (suppl.), 593-600.
  3. Wiegand T. W., Williams P. B., Dreskin S. C., Jou-vin M. H., Kinet J. P., Tasset D. J. Immunol. High-affinity oligonucleotide ligands to human IgE inhibit binding to Fc epsilon receptor I. 1996, 157 (suppl.), 221-30.
  4. Раев М. Б., Храмцов П. В., Бочкова М. С. Исследование размеров углеродных наночастиц, ковалентно функционализированных белковыми макромолекулами. Российские Нанотехнологии. 2015, 1-2, 112-118.
  5. В. П. Тимганова, М. С. Бочкова, М. Б. Раев. Стабильность структурно-функциональных свойств углеродных диагностикумов. Доклады академии наук. 2013, 450, 4, 492-495.

版权所有 © Rayev M.B., Khramtsov P.V., Bochkova M.S., Timganova V.P., Zamorina S.A., Kropaneva M.D., 2017

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