Evaluation of Mitochondrial Activity in Neural iPSC Derivatives Obtained from a Parkinson’s Disease Patient with Genetic Variant C.1492T>G of GLUD2

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Abstract

Parkinson’s disease (PD) is a progressive neurological disorder that can be inherited through pathogenic variants of certain genes. While variants of some genes have been linked to PD, the specific role of variant c.1492T>G in the GLUD2 gene in PD development remains unclear. The GLUD2 gene is located on the q arm of the X chromosome and is a pseudogene for GLUD1, which encodes for glutamate dehydrogenase type 1. Both GLUD2 and GLUD1 are involved in the regulation of glutamate levels in the brain, and their dysfunction has been linked to PD. However, more research is needed to fully understand the role of this particular variant in PD pathogenesis. Previously, induced pluripotent stem cells (iPSCs) from a patient with PD [PD40], were generated in the laboratory of developmental epigenetics at the Institute of Cytology and Genetics SB RAS. The patient is a 55-year-old man with a hemizygous genotype, carrying a pathogenic missense mutation (c.1492T>G, p.S498A, rs9697983) in the GLUD2 gene that leads to increased activity of the hGDH2 mitochondrial enzyme. In this study in patient-specific cells (iPSC-derived astrocytes and dopaminergic neurons), we have shown for the first time the proposed contribution of this variant to mitochondrial function in PD.

About the authors

D. A Sorogina

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

Email: d.sorogina@g.nsu.ru
Novosibirsk, Russia

E. V Grigor’eva

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

Novosibirsk, Russia

S. V Pavlova

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

Novosibirsk, Russia

A. A Malakhova

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

Novosibirsk, Russia

S. M Zakian

Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences

Novosibirsk, Russia

References

  1. DeMaagd G., Philip A. //Pharm. Ther. 2015. V. 40. № 8. P. 504.
  2. Lesage S., Brice A. // Hum. Mol. Genet. 2009. V. 18. № R1.
  3. Malkus K.A., Tsika E., Ischiropoulos H. // Mol. Neurodegener. 2009. Vol. 4. № 1.
  4. Klein C, Westenberger A. // Cold Spring Harb Perspect Med. 2012. V. 2. № 1.
  5. Mastorodemos V., Zaganas I., Spanaki C., Bessa M., Plaitakis A. // J. Neurosci. Res. 2005. V. 79. № 1–2. P. 65–73.
  6. Shashidharan P., Michaelidis T.M., Robakis N.K., Kresovali A., Papamatheakis J., Plaitakis A. // J. Biol. Chem. 1994. V. 269. № 24. P. 16971–16976.
  7. Plaitakis A., Latsoudis H., Spanaki C. // Neurochem. Int. 2011. V. 59. № 4. P. 495–509.
  8. Plaitakis A., Kalef-Ezra E., Kotzamani D., Zaganas I., Spanaki C. // Biology (Basel). 2017. V. 6. № 1.
  9. Mathioudakis L., Bourbouli M., Daklada E., Kargatzi S., Michaelidou K., Zaganas I. // Neurochem. Res. 2019. V. 44. № 1. P. 170–187
  10. Smith H., Li C., Stanley C., Smith T. // Neurochem. Res. 2019. V. 44. № 1. P. 117–132.
  11. Jin L., Li D., Alesi G.N., Fan J., Kang H.B., Lu Z., Boggon T.J., Jin P., Yi H., Wright E.R., Duong D., Seyfried N.T., Egnatchik R., DeBerardinis R.J., Magliocca K.R., He C., Arellano M.L., Khoury H.J., Shin D.M., Khuri F.R., Kang S. // Cancer Cell. 2015. V. 27. № 2. P. 257–270.
  12. Plaitakis A., Sidiropoulou K., Kotzamani D., Litso I., Zaganas I., Spanaki C. // Int. J. Mol. Sci. V. 25. № 10. P. 5297.
  13. Nissen J.D., Lykke K., Bryk J., Stridh M.H., Zaganas I., Skytt D.M., Schousboe A., Bak L.K., Enard W., Paabo S., Waagepetersen H.S. // Glia. 2017. V. 65. № 3. P. 474–488. https://doi.org/10.1002/glia.23105. Epub 2016 Dec 29. PMID: 28032919.
  14. Spanaki C., Sidiropoulou K., Petraki Z., Diskos K., Konstantoudaki X., Volitaki E., Mylonaki K., Savvaki M., Plaitakis A. // iScience. 2024. V. 27. № 2.
  15. Liu G., Zhu J., Yu M., Cai C., Zhou Y., Yu M., Fu Z., Gong Y., Yang B., Li Y., Zhou Q., Lin Q., Ye H., Ye L., Zhao X., Li Z., Chen R., Han F., Tang C., Zeng B. // J. Transl. Med. 2015. V. 13. P. 144.
  16. Chen R., Nishimura M.C., Kharbanda S., Peale F., Deng Y., Daemen A., Forrest WF, Kwong M, Hedehus M., Hatzivassiliou G., Friedman L.S., Phillips H.S. // Proc. Natl. Acad. Sci. USA. 2014. V. 111. № 39. P. 14217–14222.
  17. Franceschi S., Corsinovi D., Lessi F., Tantillo E., Aretini P., Menicagli M., Scopelliti C., Civita P., Pasqualetti F., Naccarato A.G., Ori M., Mazzanti C.M. // EBioMedicine. 2018. V. 37. P. 56–67.
  18. Plaitakis A., Latsoudis H., Kanavouras K., Ritz B., Bronstein J.M., Skoula I., Mastorodemos V., Papapetropoulos S., Borompokas N., Zaganas I., Xiromerisiou G., Hadjigeorgiou G.M., Spanaki C. // Eur. J. Hum. Genet. 2010. V. 18. № 3. P. 336–341.
  19. Zhang W., Gong J., Ding L., Zhang Z., Pan X., Chen X., Guo W., Zhang X., Yang X., Peng G., Lin Y., Gao F., Li Y., Zhu X., Xuan A., Wang S., Sun X., Zhang Y., Xu P. // Cell Death Dis. 2020. V. 11. № 10. P. 1–17.
  20. Sorogina D.A., Grigor’eva E.V., Malakhova A.A., Pavlova S.V., Medvedev S.P., Vyatkin Y.V., Khabarova E.A., Rzaev J.A., Zakian S.M. // RUSS. J. DEV. BIOL.+, 2023. V. 54. P. 104–111.
  21. Malakhova A.A., Grigor’eva E.V., Pavlova S.V., Malankhanova T.B., Valetdinova K.R., Vyatkin Y.V., Khabarova E.A., Rzaev J.A., Zakian S.M., Medvedev S.P. // Stem Cell Research. 2020. V. 48. P. 101952–101955.
  22. Jacquet A. D. R. // Curr. Protoc. Cell Biol. 2019. Vol. 85. № 1. P. 98.
  23. Grigor’eva E.V., Kopytova A.E., Yarkova E.S., Pavlova S.V., Sorogina D.A., Malakhova A.A., Malankhanova T.B., Baydakova G.V., Zakharova E.Y., Medvedev S.P., Pchelina S.N., Zakian S.M. // INT. J. MOL. SCI. 2023. V. 23. № 5. P. 4437.
  24. Yarkova E.S., Grigor’eva E.V., Medvedev S.P., Tarasevich D.A., Pavlova S.V., Valetdinova K.R., Minina J.M., Zakian S.M., Malakhova A.A. // Biomedicines. 2024. V. 12. № 4. P. 744.
  25. Wang M., Ling K.H., Tan J.J., Lu C.B. // Cells. 2020. V. 9. № 6. P. 1–26.
  26. Krencik R., Zhang, S.C. // Nat. Protoc. 2011. V. 6. P. 1710–1717.
  27. Spanaki C., Kotzamani D., Kleopa K., Plaitakis A. // Mol. Neurobiol. 2016. V. 53. № 8. P. 5140–5148.
  28. Spanaki C., Kotzamani D., Petraki Z., Drakos E., Plaitakis A. // Neurochem. Res. 2014. V. 39. № 3. P. 500–515.

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