Study of the Biocatalyst Granule Distribution of Yeast Cells for a Continuous Bioreactor using the 3D X-Ray Tomography Technique

Abstract

The methodology of studying the distribution of yeast cells in granules by 3D X-ray microtomography is presented. A method of cell dyeing by iodine-dodecanol solution is proposed. Granules with yeast biomass are investigated at the XRT-MT station of the Kurchatov source of synchrotron radiation. The distribution of the biomass over the granules is found. This method can be applied for other research in the field of biomass immobilization on different carrier materials used in the biotechnological industry.