大鼠小肠和结肠壁内自主神经节在出生后个体发育中的形态计量学特征
- 作者: Masliukov P.M.1, Budnik A.F.2
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隶属关系:
- Yaroslavl State Medical University
- Kabardino-Balkarian State University
- 期: 卷 163, 编号 4 (2025)
- 页面: 305-315
- 栏目: Original Study Articles
- URL: https://journals.rcsi.science/1026-3543/article/view/349035
- DOI: https://doi.org/10.17816/morph.677905
- EDN: https://elibrary.ru/GICHGI
- ID: 349035
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详细
论证。成年动物肠壁内自主神经节的形态学，尤其是肌间（myenteric plexus, MP）和黏膜下（submucosal plexus, SP）神经丛，已有较深入研究，但关于这些结构的年龄相关特征在现有文献中仍缺乏充分资料。
目的。 研究大鼠小肠和结肠壁内肌间神经丛和黏膜下神经丛自主神经节在出生后个体发育中的形态计量学特征。
方法。选用不同年龄阶段的Wistar系雄性大鼠：新生鼠，以及出生后第10、20、30、60天，及12月龄和24月龄。采用免疫组织化学方法，使用带荧光标记的抗蛋白基因产物9.5（PGP9.5）抗体进行分析。
结果。在出生后发育过程中，小肠和结肠内每1 mm2的神经节数量逐渐减少，而神经节面积逐渐增大。小肠和结肠MP神经节的平均面积自出生起增加至第60天；SP神经节的平均面积则在出生后前30天内增加。MP每mm2的神经节密度在小肠于前60天下降，在结肠于前12月内下降。SP的密度在小肠和结肠均于出生后前60天内下降。在MP，每个神经节的PGP9.5免疫反应性神经元平均数在整个发育过程中保持稳定，而在SP，该平均数在出生后前10天增加。
结论。在大鼠出生后个体发育的前30天内，MP和SP的神经节体积增大，而在小肠和结肠单位面积上的密度降低。MP的神经节形态及单个神经节内的神经元数量在发育过程中保持不变。与大鼠小肠和结肠的MP不同，出生时SP的神经节仍不成熟，其神经节网络的形成发生在出生后前10天。
作者简介
Petr M. Masliukov
Yaroslavl State Medical University
编辑信件的主要联系方式.
Email: mpm@ysmu.ru
ORCID iD: 0000-0002-6230-5024
SPIN 代码: 7676-0849
Dr. Sci. (Medicine), Professor
俄罗斯联邦, YaroslavlAntonina F. Budnik
Kabardino-Balkarian State University
Email: budnik74@mail.ru
ORCID iD: 0000-0002-3333-5865
SPIN 代码: 3691-4817
Cand. Sci. (Medicine), Assistant Professor
俄罗斯联邦, Nalchik参考
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Fig. 1. PGP9.5-immunoreactive neurons in the myenteric plexus ganglia of the small intestine in rats: a, newborn; b, postnatal day 10; c, postnatal day 30; d, 24 months of age. Cy3 fluorescence, scale bar: 50 μm.
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Fig. 2. PGP9.5-immunoreactive neurons in the submucosal plexus ganglia of the small intestine in rats: a, newborn; b, postnatal day 10; c, postnatal day 30; d, 24 months of age. Cy3 fluorescence, scale bar: 50 μm.
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Fig. 3. Diagram of changes in mean ganglion area (μm2) in the myenteric and submucosal plexuses of the small and large intestines in rats during postnatal ontogenesis. *p < 0.05 compared with the myenteric plexus of the small intestine; #p < 0.05 compared with the submucosal plexus of the small intestine.
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Fig. 4. Diagram of changes in ganglion density (N/mm2) in the myenteric and submucosal plexuses of the small and large intestines in rats during postnatal ontogenesis: MP, myenteric plexus; SP, submucosal plexus; *p < 0.05 compared with the myenteric plexus of the small intestine; #p < 0.05 compared with the submucosal plexus of the small intestine.
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Fig. 5. Diagram of changes in the number of neurons per ganglion in the myenteric and submucosal plexuses of the small and large intestines in rats during postnatal ontogenesis: MP, myenteric plexus; SP, submucosal plexus; *p < 0.05 compared with the myenteric plexus of the small intestine; #p < 0.05 compared with the submucosal plexus of the small intestine.
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Fig. 6. Diagram of changes in mean neuronal cross-sectional area in the myenteric and submucosal plexuses of the small and large intestines in rats during postnatal ontogenesis: MP, myenteric plexus; SP, submucosal plexus; *p < 0.05 compared with the myenteric plexus.
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