Dynamics of the activity of antioxidant enzymes and the expression of the genes encoding them in wheat after ultrasound exposure

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The effect of ultrasound (5, 10 and 20 min, intensity 25 kW/m2, frequency 26.1 kHz) on the dynamics of the activity of the main antioxidant enzymes: superoxide dismutase, catalase, peroxidase and the expression of the genes encoding them (SOD-1, CAT, POD) was studied in germinating seeds and wheat sprouts. Ultrasound after 1 hour predominantly suppressed the activity of antioxidant enzymes, with subsequent restoration and increase (after 1 and 6 days) of activity. The content of mRNA transcripts of the studied genes predominantly increased 1 hour after exposure, and subsequently (after 1 and 6 days) it was either higher or remained equal to the control. The results obtained apparently indicate that ultrasound triggers eustress mechanisms, i. e. the stimulating effect led to the mobilization of protective processes of cells – hormesis.

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S. Tarasov

Nizhny Novgorod State Agricultural Academy

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Email: tarasov_ss@mail.ru
俄罗斯联邦, 603022, Nizhny Novgorod, Gagarin Ave., 97

E. Krutova

Nizhny Novgorod State Agricultural Academy

Email: tarasov_ss@mail.ru
俄罗斯联邦, 603022, Nizhny Novgorod, Gagarin Ave., 97

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2. Fig. 1. Effect of duration of ultrasound exposure on the dynamics of superoxide dismutase activity (I) and the content of SOD-1 gene transcripts (II) in germinating seeds (a, b, c) and sprouts (d) of wheat. K – control; 5, 10 and 20 – duration of ultrasound exposure to seeds, min; a – immediately after exposure; b – after 1 hour; c – after 24 hours; d – after 6 days of ultrasound exposure, respectively. *P ≤ 0.05 relative to the control according to the Student’s criterion; **P ≤ 0.05 in comparison with the control according to the Kruskal-Wallis criterion.

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3. Fig. 2. Effect of the duration of ultrasound exposure on the dynamics of catalase activity (I) and the content of CAT-1 gene transcripts (II) in germinating seeds (a, b, c) and sprouts (d) of wheat. 1, 2 and 3 – time of enzyme activity fixation, min. Designations see Fig. 1.

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4. Fig. 3. Effect of duration of ultrasound exposure on the dynamics of soluble peroxidase activity (I) and the content of POD gene transcripts (II) in germinating seeds (a, b, c) and sprouts (d) of wheat. Designations see Fig. 1.

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