A Multiplex PCR Assay Mediated by Universal Primer for the Diagnosis of Human Meningitis Caused by Six Common Bacteria
- Autores: Duan Q.1, Lu S.2, Hu Y.3, Shen S.1, Xi B.1, Wang X.1, Sun W.1
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Afiliações:
- Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
- Emergency Department
- Department of Oncology
- Edição: Volume 54, Nº 4 (2018)
- Páginas: 423-430
- Seção: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/188921
- DOI: https://doi.org/10.1134/S1022795418040075
- ID: 188921
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Resumo
The present study aimed to develop a universal primer-multiplex PCR (UP-M-PCR) assay for the detection of six common bacteria associated with human meningitis. One optimal universal primer (UP) was selected from three UPs by comparing their sensitivities and specificities. All specific primers were tagged with the UP sequence at 5' end, and applied to the multiplex PCR system. The multiplex system was further optimized and assessed. This UP-M-PCR can successfully detect the six meningitis-associated pathogens with high specificity, and the sensitivity could reach up to 10 copies. In the identification of clinical specimens, six positive cases infected with Streptococcus agalactiae, Staphylococcus aureus, and Streptococcus pneumoniae were confirmed. The newly developed multiplex PCR system can be used to detect the six pathogens associated with human bacterial meningitis with high specificity and sensitivity.
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Sobre autores
Q. Duan
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
República Popular da China, Suzhou, 215123
S. Lu
Emergency Department
Email: sunwanping@suda.edu.cn
República Popular da China, Suzhou
Y. Hu
Department of Oncology
Email: sunwanping@suda.edu.cn
República Popular da China, Suzhou
S. Shen
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
República Popular da China, Suzhou, 215123
B. Xi
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
República Popular da China, Suzhou, 215123
X. Wang
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
República Popular da China, Suzhou, 215123
W. Sun
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Autor responsável pela correspondência
Email: sunwanping@suda.edu.cn
República Popular da China, Suzhou, 215123