A Multiplex PCR Assay Mediated by Universal Primer for the Diagnosis of Human Meningitis Caused by Six Common Bacteria
- Authors: Duan Q.Q.1, Lu S.Q.2, Hu Y.X.3, Shen S.N.1, Xi B.S.1, Wang X.N.1, Sun W.P.1
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Affiliations:
- Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
- Emergency Department
- Department of Oncology
- Issue: Vol 54, No 4 (2018)
- Pages: 423-430
- Section: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/188921
- DOI: https://doi.org/10.1134/S1022795418040075
- ID: 188921
Cite item
Abstract
The present study aimed to develop a universal primer-multiplex PCR (UP-M-PCR) assay for the detection of six common bacteria associated with human meningitis. One optimal universal primer (UP) was selected from three UPs by comparing their sensitivities and specificities. All specific primers were tagged with the UP sequence at 5' end, and applied to the multiplex PCR system. The multiplex system was further optimized and assessed. This UP-M-PCR can successfully detect the six meningitis-associated pathogens with high specificity, and the sensitivity could reach up to 10 copies. In the identification of clinical specimens, six positive cases infected with Streptococcus agalactiae, Staphylococcus aureus, and Streptococcus pneumoniae were confirmed. The newly developed multiplex PCR system can be used to detect the six pathogens associated with human bacterial meningitis with high specificity and sensitivity.
About the authors
Q. Q. Duan
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
China, Suzhou, 215123
S. Q. Lu
Emergency Department
Email: sunwanping@suda.edu.cn
China, Suzhou
Y. X. Hu
Department of Oncology
Email: sunwanping@suda.edu.cn
China, Suzhou
S. N. Shen
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
China, Suzhou, 215123
B. S. Xi
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
China, Suzhou, 215123
X. N. Wang
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
China, Suzhou, 215123
W. P. Sun
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Author for correspondence.
Email: sunwanping@suda.edu.cn
China, Suzhou, 215123