A Multiplex PCR Assay Mediated by Universal Primer for the Diagnosis of Human Meningitis Caused by Six Common Bacteria
- Авторлар: Duan Q.1, Lu S.2, Hu Y.3, Shen S.1, Xi B.1, Wang X.1, Sun W.1
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Мекемелер:
- Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
- Emergency Department
- Department of Oncology
- Шығарылым: Том 54, № 4 (2018)
- Беттер: 423-430
- Бөлім: Genetics of Microorganisms
- URL: https://journals.rcsi.science/1022-7954/article/view/188921
- DOI: https://doi.org/10.1134/S1022795418040075
- ID: 188921
Дәйексөз келтіру
Аннотация
The present study aimed to develop a universal primer-multiplex PCR (UP-M-PCR) assay for the detection of six common bacteria associated with human meningitis. One optimal universal primer (UP) was selected from three UPs by comparing their sensitivities and specificities. All specific primers were tagged with the UP sequence at 5' end, and applied to the multiplex PCR system. The multiplex system was further optimized and assessed. This UP-M-PCR can successfully detect the six meningitis-associated pathogens with high specificity, and the sensitivity could reach up to 10 copies. In the identification of clinical specimens, six positive cases infected with Streptococcus agalactiae, Staphylococcus aureus, and Streptococcus pneumoniae were confirmed. The newly developed multiplex PCR system can be used to detect the six pathogens associated with human bacterial meningitis with high specificity and sensitivity.
Негізгі сөздер
Авторлар туралы
Q. Duan
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
ҚХР, Suzhou, 215123
S. Lu
Emergency Department
Email: sunwanping@suda.edu.cn
ҚХР, Suzhou
Y. Hu
Department of Oncology
Email: sunwanping@suda.edu.cn
ҚХР, Suzhou
S. Shen
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
ҚХР, Suzhou, 215123
B. Xi
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
ҚХР, Suzhou, 215123
X. Wang
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Email: sunwanping@suda.edu.cn
ҚХР, Suzhou, 215123
W. Sun
Laboratory of Molecular Diagnostics, College of Pharmaceutical Sciences
Хат алмасуға жауапты Автор.
Email: sunwanping@suda.edu.cn
ҚХР, Suzhou, 215123