STENOFOLIA gene and regulation of somatic embryogenesis in Medicago truncatula


Citar

Texto integral

Acesso aberto Acesso aberto
Acesso é fechado Acesso está concedido
Acesso é fechado Somente assinantes

Resumo

Expression of STENOFOLIA (STF) gene was analyzed in different organs of line R-108 barrel medic plants (Medicago truncatula Gaertn.). Maximum levels of STF expression were observed in the generative organs, fruits and flowers, suggesting its involvement in the reproduction process. The local analysis of STF expression during somatic embryogenesis in M. truncatula showed that the STF promoter is activated directly in somatic embryos. Overexpression of STF gene in the calli of R-108 line leads to increased embryogenicity, accompanied by reduced levels of MtGH3.6 gene expression, which is responsible for auxin metabolism, and also reduced expression levels of gene encoding MtHB1 transcription factor. Comparative analysis of MtGH3.6 and MtHB1 gene expression dynamics in the calli of embryogenic 2HA line and non-embryogenic A17 line of M. truncatula showed that these genes demonstrated reduced level of expression in embryogenic calli compared to non-embryogenic. These data suggested that STF stimulates the formation of somatic embryos, directly or indirectly inhibiting the transcription of MtGH3.6, MtHB1 and, probably, many other genes.

Sobre autores

V. Tvorogova

Department of Genetics and Biotechnology

Autor responsável pela correspondência
Email: krubaza@mail.ru
Rússia, St. Petersburg, 199034

Yu. Fedorova

Department of Genetics and Biotechnology

Email: krubaza@mail.ru
Rússia, St. Petersburg, 199034

F. Zhang

Department of Plant and Soil Sciences

Email: krubaza@mail.ru
Estados Unidos da América, Ardmore, Oklahoma, 73401

L. Lutova

Department of Genetics and Biotechnology

Email: krubaza@mail.ru
Rússia, St. Petersburg, 199034

Arquivos suplementares

Arquivos suplementares
Ação
1. JATS XML

Declaração de direitos autorais © Pleiades Publishing, Ltd., 2016