Genome Instability of Hippocampal and Bone Marrow Cells in Male Mice after the Action of Immobilization and Pheromonal Stressor

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Different stressors, affecting the cells of target organs, can lead to genomic instability and even disintegration, which can play a role in the formation of post-stress pathologies. We studied the effect of psycho-emotional stressors (immobilization and mouse stress pheromone – 2,5-dimethylpyrazine) on the DNA integrity of hippocampal and bone marrow cell in male mice of CD1, CBA and C3H strains. Cytogenetic and immunocytochemical methods (alkaline comet assay, ana-telophase analysis of mitotic disturbances and analysis of γH2AX foci) were used. It is shown that the classic mouse stressor (immobilization or restraint), similar as 2,5-dimethylpyrazine, damages the genome of the cells of both organs studied. The destabilization of the cell genome of various organs is considered as an essential stage in the development of a stress response, which is an attempt of the organism to adapt to extreme environmental influences.

Sobre autores

V. Shcherbinina

Saint Petersburg State University; Pavlov Institute of Physiology Russian Academy of Sciences

Email: mouse_gene@mail.ru
Russia, Saint Petersburg; Russia, Saint Petersburg

B. Bakulevskiy

Saint Petersburg State University

Email: mouse_gene@mail.ru
Russia, Saint Petersburg

T. Glinin

Saint Petersburg State University

Email: mouse_gene@mail.ru
Russia, Saint Petersburg

E. Daev

Saint Petersburg State University; Pavlov Institute of Physiology Russian Academy of Sciences

Autor responsável pela correspondência
Email: mouse_gene@mail.ru
Russia, Saint Petersburg; Russia, Saint Petersburg

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Declaração de direitos autorais © В.Д. Щербинина, Б.В. Бакулевский, Т.С. Глинин, Е.В. Даев, 2023

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