Association of polymorphic variants of hemostatic system genes with the course of COVID-19
- Authors: Nikolaeva L.I.1, Stuchinskaya M.D.1, Dedova A.V.1, Nadezhda S.G.1, Khlopova I.N.1, Kruzhkova I.S.1,2, Merkulova L.N.1, Kisteneva L.B.1, Kolobukhina L.V.1,2, Mukasheva E.A.1, Krasnoslobodtsev K.G.1, Trushakova S.V.1, Krepkaya A.S.1, Kuprianov V.V.1, Nikitenko N.A.1, Khadorich E.A.1, Burmistrov E.M.1, Tyurin I.N.2, Antipyat N.A.2, Burtseva E.I.1
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Affiliations:
- N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
- Infection Diseases Clinical Hospital Number 1, Moscow Department of Health
- Issue: Vol 68, No 5 (2023)
- Pages: 445-453
- Section: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/231863
- DOI: https://doi.org/10.36233/0507-4088-197
- EDN: https://elibrary.ru/guttrz
- ID: 231863
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Abstract
Introduction. COVID-19 is characterized by a varied clinical course.
The aim of the work was to identify associations of SNPs of hemostatic system genes with COVID-19.
Materials and methods. DNA was isolated from patients (n=117) and healthy participants (n=104). All infected patients were divided into 3 groups, depending on disease severity assessment, which was appreciated by NEWS2. Another group consisted of participants, who had asymptomatic infection in the past. Determination of SNPs of the genes FGB (-455 G/A), FII (20210 G/A), FV (1691 G/A), FVII (10976 G/A), FXIIIA1 (103 G/T), ITGA2 (807 C/T), ITGB3 (1565 T/C), SERPINE1 (-675 5G/4G) were performed by PCR using the “Genetics of Hemostasis” kit (“DNA-Technology”, Russia).
Results. In analyzed SNPs, no significant differences were detected between the group of infected patients and healthy participants. But significant association was revealed in gene SERPINE1 (-675 5G/4G), when patient groups, differing in the disease severity, were analyzed relative to the group of participants with asymptomatic infection (p=0.0381; p=0 .0066; p=0.0009). It was found, that as COVID-19 severity scores increased, the proportion of 5G allele of gene SERPINE1 decreased, and the proportion of the 4G allele increased (p=0.005; p=0.009; p=0.0005). Similar processes were observed for genotypes 5G/5G and 4G/4G.
Discussion. The gene SERPINE1 (-675 5G/4G) is associated with the severity of COVID-19.
Conclusion. For the first time, it was discovered that 5G/5G genotype of gene SERPINE1 (-675 5G/4G) can be a marker of a milder course of COVID-19, and the 4G/4G genotype as a more severe one.
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##article.viewOnOriginalSite##About the authors
Lyudmila I. Nikolaeva
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Author for correspondence.
Email: l.i.nikolaeva@mail.ru
ORCID iD: 0000-0002-1323-5568
D.Sci. in Biology, Leading Researcher of the Laboratory Gene Engineering Products
Russian Federation, 123098, MoscowMaya D. Stuchinskaya
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: mayastaya@mail.ru
ORCID iD: 0000-0001-8544-7482
junior researcher of the Laboratory Gene Engineering Products
Russian Federation, 123098, MoscowAnna V. Dedova
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: dedova.anna2010@mail.ru
ORCID iD: 0000-0002-2491-9324
graduate student of the Laboratory Gene Engineering Products
Russian Federation, 123098, MoscowShevchenko G. Nadezhda
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: dr.nadya@inbox.ru
ORCID iD: 0000-0002-2486-4554
junior researcher of the Laboratory Gene Engineering Products
Russian Federation, 123098, MoscowIrina N. Khlopova
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: khlopova.ira@yandex.ru
ORCID iD: 0000-0002-7419-590X
C.Sci. in Medicine, leading researcher of the Laboratory chronic viral infections
Russian Federation, 123098 MoscowIrina S. Kruzhkova
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia; Infection Diseases Clinical Hospital Number 1, Moscow Department of Health
Email: irina-kru@yandex.ru
ORCID iD: 0000-0002-1983-481X
researcher of the Laboratory respiratory viral infections with drug testing
Russian Federation, 123098 Moscow; 125367, MoscowLilya N. Merkulova
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: merkulova0320@yandex.ru
ORCID iD: 0000-0002-7260-0879
C.Sci. in Medicine, leading researcher of the Laboratory respiratory of the viral infections with drug testing
Russian Federation, 123098, MoscowLidya B. Kisteneva
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: lborisovna2007@yandex.ru
ORCID iD: 0000-0001-7336-409X
D.Sci. in Medicine, leading researcher of the Laboratory chronic viral infections
Russian Federation, 123098, MoscowLyudmila V. Kolobukhina
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia; Infection Diseases Clinical Hospital Number 1, Moscow Department of Health
Email: lkolobuchina@yandex.ru
ORCID iD: 0000-0001-5775-3343
D.Sci. in Medicine, leading researcher of the Laboratory respiratory viral infections with drug testing
Russian Federation, 123098 Moscow; 125367, MoscowEvgenya A. Mukasheva
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: mukasheva_evgeniya@mail.ru
ORCID iD: 0000-0002-5688-5309
researcher of the Laboratory of Influenza Etiology and Epidemiology
Russian Federation, 123098, MoscowKirill G. Krasnoslobodtsev
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: kkg_87@mail.ru
ORCID iD: 0000-0003-1745-9128
researcher of the Laboratory of Influenza Etiology and Epidemiology
Russian Federation, 123098, MoscowSvetlana V. Trushakova
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: s.trushakova@gmail.com
ORCID iD: 0000-0002-9610-3041
C.Sci. in Biology, senior researcher of the Laboratory of Influenza Etiology and Epidemiology
Russian Federation, 123098, MoscowAnastasia S. Krepkaya
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: nastya18-96@mail.ru
ORCID iD: 0000-0002-7272-4011
junior researcher of the Laboratory of Influenza Etiology and Epidemiology
Russian Federation, 123098, MoscowVictor V. Kuprianov
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: vkoup@mail.ru
ORCID iD: 0000-0002-8602-1974
C.Sci. in Biology, senior researcher of the Laboratory Gene Engineering Products
Russian Federation, 123098, MoscowNatalia A. Nikitenko
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: nan-nikitenko@yandex.ru
ORCID iD: 0000-0001-5829-744X
C.Sci. in Biology, senior researcher of the Cell Microbiology Laboratory
Russian Federation, 123098, MoscowElizaveta A. Khadorich
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: eli.hadd@yandex.ru
ORCID iD: 0009-0005-1816-0496
laboratory assistant of the Cell Microbiology Laboratory
Russian Federation, 123098, MoscowEgor M. Burmistrov
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: burmistrov.gamaleya@yandex.ru
ORCID iD: 0000-0002-6592-8331
researcher of the Cell Microbiology Laboratory
Russian Federation, 123098, MoscowIgor N. Tyurin
Infection Diseases Clinical Hospital Number 1, Moscow Department of Health
Email: tyurin.dti@yandex.ru
ORCID iD: 0000-0002-5696-1586
C.Sci. in Medicine, Chief Physician, Infection Diseases Clinical Hospital No 1, Moscow Department of Health
Russian Federation, 125367, MoscowNatalia A. Antipyat
Infection Diseases Clinical Hospital Number 1, Moscow Department of Health
Email: natadoc70@bk.ru
ORCID iD: 0000-0001-8578-2838
Deputy Chief Physician for Medical Affairs, Infection Diseases Clinical Hospital No 1, Moscow Department of Health
Russian Federation, 125367, MoscowElena I. Burtseva
N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia
Email: elena-burtseva@yandex.ru
ORCID iD: 0000-0003-2518-6801
D.Sci. in Medicine, Head of the Laboratory of Influenza Etiology and Epidemiology
Russian Federation, 123098, MoscowReferences
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