Association of polymorphic variants of hemostatic system genes with the course of COVID-19

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Abstract

Introduction.  COVID-19 is characterized by a varied clinical course.

The aim of the work was to identify associations of SNPs of hemostatic system genes with COVID-19.

Materials and methods. DNA was isolated from patients (n=117) and healthy participants (n=104). All infected patients were divided into 3 groups, depending on disease severity assessment, which was appreciated by NEWS2. Another group consisted of participants, who had asymptomatic infection in the past. Determination of SNPs of the genes FGB (-455 G/A), FII (20210 G/A), FV (1691 G/A), FVII (10976 G/A), FXIIIA1 (103 G/T), ITGA2 (807 C/T), ITGB3 (1565 T/C), SERPINE1 (-675 5G/4G) were performed by PCR using the “Genetics of Hemostasis” kit (“DNA-Technology”, Russia).

Results. In analyzed SNPs, no significant differences were detected between the group of infected patients and healthy participants. But significant association was revealed in gene SERPINE1 (-675 5G/4G), when patient groups, differing in the disease severity, were analyzed relative to the group of participants with asymptomatic infection (p=0.0381; p=0 .0066; p=0.0009). It was found, that as COVID-19 severity scores increased, the proportion of 5G allele of gene SERPINE1 decreased, and the proportion of the 4G allele increased (p=0.005; p=0.009; p=0.0005). Similar processes were observed for genotypes 5G/5G and 4G/4G.

Discussion. The gene SERPINE1 (-675 5G/4G) is associated with the severity of COVID-19.

Conclusion. For the first time, it was discovered that 5G/5G genotype of gene SERPINE1 (-675 5G/4G) can be a marker of a milder course of COVID-19, and the 4G/4G genotype as a more severe one.

About the authors

Lyudmila I. Nikolaeva

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Author for correspondence.
Email: l.i.nikolaeva@mail.ru
ORCID iD: 0000-0002-1323-5568

D.Sci. in Biology, Leading Researcher of the Laboratory Gene Engineering Products

Russian Federation, 123098, Moscow

Maya D. Stuchinskaya

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: mayastaya@mail.ru
ORCID iD: 0000-0001-8544-7482

junior researcher of the Laboratory Gene Engineering Products

Russian Federation, 123098, Moscow

Anna V. Dedova

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: dedova.anna2010@mail.ru
ORCID iD: 0000-0002-2491-9324

graduate student of the Laboratory Gene Engineering Products

Russian Federation, 123098, Moscow

Shevchenko G. Nadezhda

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: dr.nadya@inbox.ru
ORCID iD: 0000-0002-2486-4554

junior researcher of the Laboratory Gene Engineering Products

Russian Federation, 123098, Moscow

Irina N. Khlopova

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: khlopova.ira@yandex.ru
ORCID iD: 0000-0002-7419-590X

C.Sci. in Medicine, leading researcher of the Laboratory chronic viral infections

Russian Federation, 123098 Moscow

Irina S. Kruzhkova

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia; Infection Diseases Clinical Hospital Number 1, Moscow Department of Health

Email: irina-kru@yandex.ru
ORCID iD: 0000-0002-1983-481X

researcher of the Laboratory respiratory viral infections with drug testing

Russian Federation, 123098 Moscow; 125367, Moscow

Lilya N. Merkulova

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: merkulova0320@yandex.ru
ORCID iD: 0000-0002-7260-0879

C.Sci. in Medicine, leading researcher of the Laboratory respiratory of the viral infections with drug testing

Russian Federation, 123098, Moscow

Lidya B. Kisteneva

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: lborisovna2007@yandex.ru
ORCID iD: 0000-0001-7336-409X

D.Sci. in Medicine, leading researcher of the Laboratory chronic viral infections

Russian Federation, 123098, Moscow

Lyudmila V. Kolobukhina

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia; Infection Diseases Clinical Hospital Number 1, Moscow Department of Health

Email: lkolobuchina@yandex.ru
ORCID iD: 0000-0001-5775-3343

D.Sci. in Medicine, leading researcher of the Laboratory respiratory viral infections with drug testing

Russian Federation, 123098 Moscow; 125367, Moscow

Evgenya A. Mukasheva

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: mukasheva_evgeniya@mail.ru
ORCID iD: 0000-0002-5688-5309

researcher of the Laboratory of Influenza Etiology and Epidemiology

Russian Federation, 123098, Moscow

Kirill G. Krasnoslobodtsev

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: kkg_87@mail.ru
ORCID iD: 0000-0003-1745-9128

researcher of the Laboratory of Influenza Etiology and Epidemiology

Russian Federation, 123098, Moscow

Svetlana V. Trushakova

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: s.trushakova@gmail.com
ORCID iD: 0000-0002-9610-3041

C.Sci. in Biology, senior researcher of the Laboratory of Influenza Etiology and Epidemiology

Russian Federation, 123098, Moscow

Anastasia S. Krepkaya

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: nastya18-96@mail.ru
ORCID iD: 0000-0002-7272-4011

junior researcher of the Laboratory of Influenza Etiology and Epidemiology

Russian Federation, 123098, Moscow

Victor V. Kuprianov

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: vkoup@mail.ru
ORCID iD: 0000-0002-8602-1974

C.Sci. in Biology, senior researcher of the Laboratory Gene Engineering Products

Russian Federation, 123098, Moscow

Natalia A. Nikitenko

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: nan-nikitenko@yandex.ru
ORCID iD: 0000-0001-5829-744X

C.Sci. in Biology, senior researcher of the Cell Microbiology Laboratory

Russian Federation, 123098, Moscow

Elizaveta A. Khadorich

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: eli.hadd@yandex.ru
ORCID iD: 0009-0005-1816-0496

laboratory assistant of the Cell Microbiology Laboratory

Russian Federation, 123098, Moscow

Egor M. Burmistrov

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: burmistrov.gamaleya@yandex.ru
ORCID iD: 0000-0002-6592-8331

researcher of the Cell Microbiology Laboratory

Russian Federation, 123098, Moscow

Igor N. Tyurin

Infection Diseases Clinical Hospital Number 1, Moscow Department of Health

Email: tyurin.dti@yandex.ru
ORCID iD: 0000-0002-5696-1586

C.Sci. in Medicine, Chief Physician, Infection Diseases Clinical Hospital No 1, Moscow Department of Health

Russian Federation, 125367, Moscow

Natalia A. Antipyat

Infection Diseases Clinical Hospital Number 1, Moscow Department of Health

Email: natadoc70@bk.ru
ORCID iD: 0000-0001-8578-2838

Deputy Chief Physician for Medical Affairs, Infection Diseases Clinical Hospital No 1, Moscow Department of Health

Russian Federation, 125367, Moscow

Elena I. Burtseva

N.F. Gamaleya National Research Center of Epidemiology and Microbiology of the Ministry of Health of Russia

Email: elena-burtseva@yandex.ru
ORCID iD: 0000-0003-2518-6801

D.Sci. in Medicine, Head of the Laboratory of Influenza Etiology and Epidemiology

Russian Federation, 123098, Moscow

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Copyright (c) 2023 Nikolaeva L.I., Stuchinskaya M.D., Dedova A.V., Nadezhda S.G., Khlopova I.N., Kruzhkova I.S., Merkulova L.N., Kisteneva L.B., Kolobukhina L.V., Mukasheva E.A., Krasnoslobodtsev K.G., Trushakova S.V., Krepkaya A.S., Kuprianov V.V., Nikitenko N.A., Khadorich E.A., Burmistrov E.M., Tyurin I.N., Antipyat N.A., Burtseva E.I.

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