Study of the antiviral activity of the liquid corneal storage medium in relation to the herpes simplex virus in vitro

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Abstract

The aim of the study was to assess the antiviral activity of the 7 types of liquid corneal storage medium on an experimental model of herpesvirus infection in vitro.

Material and methods. The study of antiviral activity of 7 samples of liquid corneal storage medium on a model of herpesvirus infection caused by the herpes simplex virus type 1 in Vero-cell using virological and statistical research methods was carried out.

Results and discussion. All the studied images of the corneal storage medium, including the Borzenka-Moroz base medium, did not have a cytotoxic effect on Vero cell culture. Out of of 7 samples of liquid corneal storage medium, 4 samples had reliable antiviral activity against HSV-1 when used under the therapeutic regimen (1 hour after infection) and under the preventive regimen (2 hours before infection). Antiviral activity was established in 2 samples containing the interferon inducer cycloferon at a concentration of 10 mg/kg and 30 mg/kg (sample 2, 3), in a sample containing the interferon inducer gamapren 15 mg/kg (sample 5), and in a sample containing a combination of drugs - 10 mg/kg cycloferon and an acyclic nucleoside analog-acyclovir 10 mg/kg (sample 6). According to the results of 2 test regimens, the maximum statistically significant inhibitory effect in relation to HSV-1 was detected in sample 6, containing a combination of drugs. Against the background of sample 6, the infectious activity of the test virus decreased by an average of 3.2 lg, the inhibition coefficient was 54.5%.

Conclusion. The results of the study indicate the prospects of using types of media with antiviral activity (samples 2, 3, 5, 6) for storing donor corneas in order to increase the effectiveness of keratoplasty in patients with ophthalmic herpes.

About the authors

L. M. Alimbarova

National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya

Author for correspondence.
Email: virology@mail.ru
ORCID iD: 0000-0002-8972-3111

Lyudmila M. Alimbarova – Cand. Med. Sci., assistant professor, leading researcher.

Moscow, 123098

Russian Federation

T. Z. Kerimov

A.I. Evdokimov Moscow State University of Medicine and Dentistry

ORCID iD: 0000-0001-8967-6370

Timur Z. Kerimov – postgraduate of the Eye Disease Department.

Moscow, 127473

Russian Federation

S. A. Borzenok

S. Fyodorov Eye Microsurgery Complex Federal State Institution

ORCID iD: 0000-0001-9160-6240

Sergei A. Borzenok – Dr. Med. Sci., Acad. of RANS, Professor of the Eye Disease Department, A.I. Yevdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health; Head of the Center for Fundamental and Applied Biomedical Problems of S.N. Fedorov Eye Microsurgery Federal State Institution of the Ministry of Health.

Moscow, 127486

Russian Federation

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Copyright (c) 2020 Alimbarova L.M., Kerimov T.Z., Borzenok S.A.

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