COMPARISON OF RESULTS OBTAINED BY ELISA AND NEUTRALIZATION TEST IN ASSESSING THE PROTECTION OF POPULATION FROM TICK-BORNE ENCEPHALITIS
- Authors: Chernokhaeva L.L.1, Maikova G.B.1, Rogova Y.V.1, Romanenko V.V.2, Ankudinova A.V.3, Kilyachina A.S.2, Vorovich M.F.1,4, Karganova G.G.1,4
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Affiliations:
- M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides
- Center of Hygiene and Epidemiology in Sverdlovsk Region
- Ekaterinburg Research Institute of Viral Infections
- I.M. Sechenov First Moscow State Medical University
- Issue: Vol 63, No 1 (2018)
- Pages: 36-40
- Section: ORIGINAL RESEARCH
- URL: https://journals.rcsi.science/0507-4088/article/view/117963
- DOI: https://doi.org/10.18821/0507-4088-2018-63-1-36-40
- ID: 117963
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Full Text
Abstract
The enzyme-linked immunosorbent assay (ELISA) and the neutralization test (NT) are often used to determine the level of seropositive population and to evaluate the immunogenicity of vaccines. ELISA provides information on the total pool of antiviral antibodies, while NT allows the antiviral protection level of a person to be estimated. It is assumed that the 1:100 titer in ELISA and the 1:10 titer in NT are protective. Obviously, the ratio of the total pool and virus neutralizing antibodies can vary as a result of natural immunization or vaccination. In this study, two methods were used to study the blood serum samples taken in a group of inhabitants of the Sverdlovsk region aged from 1 to 60 years. The samples were collected before immunization and 30 days after two immunizations with inactivated vaccines against tick-borne encephalitis of different manufacturers. Immunizations were performed either according to a standard scheme (30-day interval between immunizations), or according to an emergency scheme (14-day interval). It was shown that the data on the presence of antiviral antibodies in protective titers obtained by ELISA and NT were consistent in more than 85% of cases. The discrepancies between the data are due, in the first place, to the difference in the sensitivities of the two methods. The proportion of seropositive people according to NT data is always greater than that according to the results of ELISA. Nevertheless, among 174 children, about 5% of recipients after a double immunization were seropositive according to ELISA, but did not have neutralizing antibodies in protective titers.
About the authors
L. L. Chernokhaeva
M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides
Author for correspondence.
Email: noemail@neicon.ru
Russian Federation
G. B. Maikova
M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides
Email: noemail@neicon.ru
Russian Federation
Yu. V. Rogova
M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides
Email: noemail@neicon.ru
Russian Federation
V. V. Romanenko
Center of Hygiene and Epidemiology in Sverdlovsk Region
Email: noemail@neicon.ru
Russian Federation
A. V. Ankudinova
Ekaterinburg Research Institute of Viral Infections
Email: noemail@neicon.ru
Russian Federation
A. S. Kilyachina
Center of Hygiene and Epidemiology in Sverdlovsk Region
Email: noemail@neicon.ru
Russian Federation
M. F. Vorovich
M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides; I.M. Sechenov First Moscow State Medical University
Email: noemail@neicon.ru
Russian Federation
G. G. Karganova
M.P. Chumakov Institute of Poliomyelitis and Viral Encephalitides; I.M. Sechenov First Moscow State Medical University
Email: karganova@bk.ru
Russian Federation
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