Clinical application of active aspiration fluid biopsy for pancreatic neoplasia diagnosis in oncology practice

A. A. Kashintcev; Кашинцев А. А; N. Yu. Kokhanenko; Коханенко Н. Ю; K. V. Pavelets; Павелец К. В; I. A. Soloviev; Соловьев И. А; D. S. Rusanov; Русанов Д. С; O. G. Vavilova; Вавилова О. Г; Y. V. Radionov; Радионов Ю. В; M. S. Dinikin; Диникин М. С; M. Yu. Agapov; Агапов М. Ю; Y. V. Pelipas; Пелипась Ю. В; Y. A. Gulyaev; Гуляев Ю. А; T. Sh. Morgoshiia; Моргошия Т. Ш; F. A. Magomedova; Магомедова Ф. А; L. A. Solovyova; Соловьева Л. А; A. A. Nadeeva; Надеева А. А; A. V. Borovikova; Боровикова А. В; K. V. Laptev; Лаптев К. В; A. V. Lemeshev; Лемешев А. В; V. Yu. Proutski; Пруцкий В. Ю

doi:10.37469/0507-3758-2025-71-6-OF-2340

Clinical application of active aspiration fluid biopsy for pancreatic neoplasia diagnosis in oncology practice

Authors: Kashintcev A.A.¹^,2, Kokhanenko N.Y.¹, Pavelets K.V.¹^,3, Soloviev I.A.³, Rusanov D.S.¹, Vavilova O.G.¹, Radionov Y.V.¹, Dinikin M.S.⁴, Agapov M.Y.⁴, Pelipas Y.V.⁴, Gulyaev Y.A.⁴, Morgoshiia T.S.¹, Magomedova F.A.¹, Solovyova L.A.¹, Nadeeva A.A.², Borovikova A.V.¹^,3, Laptev K.V.³, Lemeshev A.V.¹, Proutski V.Y.²
Affiliations:
1. Saint Petersburg State Pediatric Medical University
2. Pandx LLC
3. SPb SBIH “Mariinskyi hospital”
4. SPb SBIH “City Clinical Oncology Dispensary”
Issue: Vol 71, No 6 (2025)
Pages: 1414-1422
Section: PRACTICES OF ONCOLOGICAL INSTITUTIONS
URL: https://journals.rcsi.science/0507-3758/article/view/380322
DOI: https://doi.org/10.37469/0507-3758-2025-71-6-OF-2340
ID: 380322

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Abstract

Introduction. Differential diagnosis of pancreatic cystic neoplasms (PCNs) presents a significant clinical challenge due to their structural heterogeneity. Pancreatic juice represents a highly specific biological medium of particular interest, containing both cellular components and potential molecular biomarkers. We present a novel technique — Active Duodenal Aspiration Biopsy (DAB) — enabling collection of pancreatic juice-bile mixtures for diagnostic analysis. Aim. To evaluate the efficacy of this original DAB technique when integrated with standard diagnostic modalities across different levels of oncology care institutions. Materials and Methods. Patients with inconclusive standard diagnostic workups underwent DAB procedures. Aspirates were analyzed cytologically and through carcinoembryonic antigen (CEA) level quantification. Results. In a case series of six patients with various pancreatic cystic neoplasms, DAB demonstrated feasibility and diagnostic utility when combined with conventional verification methods. CEA levels and cytological findings from DAB aspirates enabled precise diagnosis and informed treatment strategy modifications. Conclusion. This preliminary experience indicates DAB is both safe and effective. The technique is applicable across outpatient and inpatient oncology settings and shows potential for improving early pancreatic cancer detection.

Keywords

pancreatic cancer, cystic lesion, acute pancreatitis, pancreatic juice

References

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Clinical application of active aspiration fluid biopsy for pancreatic neoplasia diagnosis in oncology practice

Full Text

Abstract

Keywords

About the authors

References

Supplementary files