Precision-cut Lung Slices Cultivation: New Approaches to Lung Development, Functioning and Pathogenesis

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Abstract

Precision-cut lung slices (PCLS) is a modern approach used to study inter- and intracellular mechanisms in lungs ex vivo, with minimal disruption to the overall cellular architecture of the tissue. The PCLS method has both advantages and disadvantages in comparison with other methods of studying lung cells. This review focused on contemporary research in lung cell biology, using the PCLS method. The objective is to emphasize new possibilities and potential applications of this method for studying the respiratory system under both normal and pathological conditions.

About the authors

A. A Volozhinskaia

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: volozhinskayaalexsandra@mail.ru
ORCID iD: 0009-0008-3038-9172
Moscow, Russian Federation

I. A Govorova

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: ischenko.i.a@gmail.com
ORCID iD: 0000-0001-5107-0621
Moscow, Russian Federation

Y. A Novikova

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: yula1308@mail.ru
ORCID iD: 0000-0003-3744-9111
Moscow, Russian Federation

E. A Vorotelyak

Koltzov Institute of Developmental Biology, Russian Academy of Sciences

Email: vorotelyak@idbras.ru
ORCID iD: 0000-0001-5405-0212
Moscow, Russian Federation

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