Alternative Chromophore Binding Sites in Non-Covalent Fluorescent Complexes of Bacterial Lipocalin

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The three-dimensional structure of DiB3, a fluorescent non-covalent complex of a genetically engineered variant of the bacterial protein lipocalin Blc with a synthetic GFP-like chromophore M739, was determined by the calculation method of molecular mechanics. Compared to the crystal structure of the related DiB1 complex, an alternative binding site for the M739 chromophore was found in the structure of the DiB3 complex.

作者简介

E. Goryacheva

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

编辑信件的主要联系方式.
Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

I. Artemyev

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

S. Arhipova

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

A. Rossohin

Research Center of Neurology

Email: goryacheva@ibch.ru
Russia, 105064, Moscow, per. Obuha 5

A. Gilvanov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

V. Pletnev

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

N. Pletnevа

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Email: goryacheva@ibch.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

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版权所有 © Е.А. Горячева, И.В. Артемьев, С.Ф. Архипова, А.В. Россохин, А. Р. Гильванов, В.З. Плетнев, Н.В. Плетнева, 2023

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