Biological Microarray for Detection of Somatic Mutations in the Genes of Isocitrate Dehydrogenase 1 and 2
- 作者: Varachev V.1, Guskov D.1, Shekhtman A.2, Rogozhin D.2, Polyakov S.1, Chudinov A.1, Zasedatelev A.1, Nasedkina T.1
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隶属关系:
- Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Russian Children’s Clinical Hospital
- 期: 卷 49, 编号 5 (2023)
- 页面: 537-542
- 栏目: ПИСЬМА РЕДАКТОРУ
- URL: https://journals.rcsi.science/0132-3423/article/view/139260
- DOI: https://doi.org/10.31857/S0132342323050093
- EDN: https://elibrary.ru/ABXFZT
- ID: 139260
如何引用文章
详细
Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) genes are important diagnostic and prognostic markers in a number of tumors (chondroid tumors, brain tumors, leukemia). To determine mutations in the IDH1/2 genes the method of allele-specific hybridization on a biological microarray was developed, which makes it possible to determine the presence of a mutation in a tumor sample (chondroid tumors and glioblastoma) and the variant of nucleotide substitution. When comparing IDH1/2 mutation detection methods using a biological microarray and DNA melting analysis with a TaqMan probe, full coincidence of results was obtained, with the biochip method making it possible to determine the type of nucleotide substitution without an additional sequencing step.
作者简介
V. Varachev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: tanased06@rambler.ru
Russia, 119991, Moscow, ul. Vavilova 32
D. Guskov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: tanased06@rambler.ru
Russia, 119991, Moscow, ul. Vavilova 32
A. Shekhtman
Russian Children’s Clinical Hospital
Email: tanased06@rambler.ru
Russia, 119117, Moscow, Leninsky prosp. 117
D. Rogozhin
Russian Children’s Clinical Hospital
Email: tanased06@rambler.ru
Russia, 119117, Moscow, Leninsky prosp. 117
S. Polyakov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: tanased06@rambler.ru
Russia, 119991, Moscow, ul. Vavilova 32
A. Chudinov
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: tanased06@rambler.ru
Russia, 119991, Moscow, ul. Vavilova 32
A. Zasedatelev
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: tanased06@rambler.ru
Russia, 119991, Moscow, ul. Vavilova 32
T. Nasedkina
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: tanased06@rambler.ru
Russia, 119991, Moscow, ul. Vavilova 32
参考
- Dang L., White D.W., Gross S., Bennett B.D., Bittinger M.A., Driggers E.M., Fantin V.R., Jang H.G., Jin S., Keenan M.C., Marks K.M., Prins R.M., Ward P.S., Yen K.E., Liau L.M., Rabinowitz J.D., Cantley L.C., Thompson C.B., Vander Heiden M.G., Su S.M. // Nature. 2009. V. 462. P. 739–744. https://doi.org/10.1038/nature08617
- Cairns R.A., Mak T.W. // Cancer Discov. 2013. V. 3. P. 730–741. https://doi.org/10.1158/2159-8290.CD-13-0083
- Chen S., Fritchie K., Wei S., Ali N., Curless K., Shen T., Brini A.T., Latif F., Sumathi V., Siegal G.P., Cheng L. // Hum. Pathol. 2017. V. 65. P. 239–246. https://doi.org/10.1016/j.humpath.2017.05.015
- Hartmann C., Meyer J., Balss J., Capper D., Mueller W., Christians A., Felsberg J., Wolter M., Mawrin C., Wick W., Weller M., Herold-Mende C., Unterberg A., Jeuken J.W., Wesseling P., Reifenberger G., von Deimling A. // Acta Neuropathol. 2009. V. 118. P. 469–474. https://doi.org/10.1007/s00401-009-0561-9
- Agarwal S., Sharma M.C., Jha P., Pathak P., Suri V., Sarkar C., Chosdol K., Suri A., Kale S.S., Mahapatra A.K., Jha P. // Neuro Oncol. 2013. V. 15. P. 718–726. https://doi.org/10.1093/neuonc/not015
- Arita H., Narita Y., Matsushita Y., Fukushima S., Yoshida A., Takami H., Miyakita Y., Ohno M., Shibui S., Ichimura K. // Brain Tumor Pathol. 2015. V. 32. P. 22–30. https://doi.org/10.1007/s10014-014-0186-0
- Perizzolo M., Winkfein B., Hui S., Krulicki W., Chan J.A., Demetrick D.J. // Brain Pathol. 2012. V. 22. P. 619–624. https://doi.org/10.1111/j.1750- 3639.2012.00579.x
- Табаков Д.В., Катаргин А.Н., Строганова А.М., Сендерович А.И., Насхлеташвили Д.Р., Киселева Н.П. // Усп. мол. онкол. 2017. Т. 4. С. 53–59. https://doi.org/10.17650/2313-805X-2017-4-1-53-59
- Emelyanova M., Arkhipova K., Mazurenko N., Chudinov A., Demidova I., Zborovskaya I., Lyubchenko L., Zasedatelev A., Nasedkina T. // Appl. Immunohistochem. Mol. Morphol. 2015. V. 23. P. 255–265. https://doi.org/10.1097/PAI.0000000000000084
- Emelyanova M., Ghukasyan L., Abramov I., Ryabaya O., Stepanova E., Kudryavtseva A., Sadritdinova A., Dzhumakova C., Belysheva T., Surzhikov S., Lyubchenko L., Zasedatelev A., Nasedkina T. // Oncotarget. 2017. V. 8. P. 52304–52320. https://doi.org/10.18632/oncotarget.17014
- Lee D., Suh Y.L., Kang S.Y., Park T.I., Jeong J.Y., Kim S.H. // Brain Pathol. 2013. V. 23. P. 285–293. https://doi.org/10.1111/bpa.12000
- Emelyanova M.A., Telysheva E.N., Orlova K.V., Ryabaya O.O., Snigiryova G.P., Abramov I.S., Mikhailovich V.M. // Cancer Genet. 2021. V. 250–251. P. 25–35. https://doi.org/10.1016/j.cancergen.2020.11.005
- Gryadunov D.A., Shaskolskiy B.L., Nasedkina T.V., Rubina A.Y., Zasedatelev A.S. // Acta Naturae. 2018. V. 10. P. 4–18. https://doi.org/10.32607/20758251-2018-10-4-4-18