Methods for Interactome Analysis of Microproteins Encoded by Small Open Reading Frames

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Abstract

Recent studies have shown that small open reading frames (sORFs, <100 codons) can encode peptides or microproteins that perform important functions in prokaryotic and eukaryotic cells. It has been established that sORF translation products are involved in the regulation of many processes, for example, they modulate the activity of the mitochondrial respiratory chain or the functions of muscle cells in mammals. However, the identification and subsequent functional analysis of peptides or microproteins encoded by sORFs is a non-trivial task and requires the use of special approaches. One of the critical steps in functional analysis is identification of protein partners of the peptide under study. This review considers the features of the interactome analysis of short protein molecules and describes the approaches currently used for studies in the field.

About the authors

I. A. Sedlov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS

Email: feigor@yandex.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

I. A. Fesenko

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS

Author for correspondence.
Email: feigor@yandex.ru
Russia, 117997, Moscow, ul. Miklukho-Maklaya 16/10

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