Assessment of the biological quality of riverine water using pathogenicity islands (PAIs) of coliform bacteria as pollution indicator

Abstract

The biological methods of quality assessment of water include the determination of harmful biological entities. In this study, quality of water from two riverine study sites was monitored in terms of the most probable number (MPN index) and pathogenicity. MPN index was found to be 0.11 and 0.15/100 mL which was also confirmed by membrane filtration technique (MFT). The isolated pure cultures were subjected to various pathogenicity tests including blood haemolysis and biofilm formation. Out of ten isolates, six showed positive results and these potentially pathogenic isolates were found to be resistant to various antibiotics like erythromycin (E), vancomycin (VA), chloramphenicol (C), tetracyclin (TE), acidoclav (AC) and kanamycin (K). Genetic analysis of these six isolates confirms the presence of flanking regions of virulence cassettes pathogenicity island (PAI) in their genome. Hence, in addition to the MPN test, the pathogenicity tests using the amplification of flanking regions of PAI can be used as a marker for determination of biological quality of water.