Apoptosis and the processes of DNA fragmentation in native and cryopreserved human sperm cells with normo- and pathosperma

Resumo

Cryopreserved human sperm is widely applied in the treatment of infertility using methods of assisted reproductive technologies. Comprehensive studies of the sperm condition after cryopreservation and subsequent cultivation will update the efficiency of the programs based on assisted reproductive technologies. The aim of this study was to investigate the effect of cryopreservation factors on the state of DNA and apoptotic processes in isolated human spermatozoa fractions in normo- and pathospermia. The analysis of DNA fragmentation and the assessment of apoptotic processes (human spermatozoa necrosis) were performed using the sperm chromatin dispersion test and the flow cytometry method. According to our study, sperm cryopreservation has not affected the DNA fragmentation index in normozoospermia and significantly increased it in oligoastenoteratozoospermia. The postcultivation increase of apoptotic and necrotic processes in oligoastenoteratozoospermia gives reason for choosing an alternative technique for oocyte fertilization— intracytoplasmic sperm injection.