Effect of parathyroid hormone on prechondroblast differentiation
- Авторлар: Torgomyan A.1, Adamyan S.1, Ghambaryan H.1, Hasratyan H.1, Khudaverdyan D.1, Archer C.2
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Мекемелер:
- Heratci State Medical University
- Swansea University
- Шығарылым: Том 50, № 5 (2016)
- Беттер: 300-303
- Бөлім: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/173436
- DOI: https://doi.org/10.3103/S0095452716050145
- ID: 173436
Дәйексөз келтіру
Аннотация
Parathyroid hormone (PTH) exerts an anabolic effect that is manifested upon periodic exposure to the hormone and a catabolic effect that is observed in case of a prolonged uninterrupted exposure to high doses of the hormone. The latter effect is characteristic of a range of pathologies, such as the hyperfunction of the parathyroid glands. The analysis of the effects of periodic and continuous administration of PTH fragment 1–34 on the differentiation of bovine prechondroblasts maintained in chondrogenic and osteogenic media was the aim of the present study. Alcian blue staining of monolayers of cultured cells maintained in a chondrogenic medium revealed the stimulation of chondrogenesis (manifested as higher intensity of matrix staining) in the case of continuous PTH administration. Alizarin red staining of monolayers of cultured cells maintained in an osteogenic medium revealed more intensive mineralization of the matrix in plates with cells that were exposed to PTH in a periodical manner. The results of the histochemical study were confirmed using electrophoretic detection of osteocrin and type II collagen.
Негізгі сөздер
Авторлар туралы
A. Torgomyan
Heratci State Medical University
Хат алмасуға жауапты Автор.
Email: adelinatorgomyan@yahoo.com
Армения, ul. Koryun 2, Yerevan
S. Adamyan
Heratci State Medical University
Email: adelinatorgomyan@yahoo.com
Армения, ul. Koryun 2, Yerevan
H. Ghambaryan
Heratci State Medical University
Email: adelinatorgomyan@yahoo.com
Армения, ul. Koryun 2, Yerevan
H. Hasratyan
Heratci State Medical University
Email: adelinatorgomyan@yahoo.com
Армения, ul. Koryun 2, Yerevan
D. Khudaverdyan
Heratci State Medical University
Email: adelinatorgomyan@yahoo.com
Армения, ul. Koryun 2, Yerevan
C. Archer
Swansea University
Email: adelinatorgomyan@yahoo.com
Ұлыбритания, Singleton Park, Swansea, Wales, SA2 8PP