Effect of parathyroid hormone on prechondroblast differentiation
- Authors: Torgomyan A.1, Adamyan S.1, Ghambaryan H.1, Hasratyan H.1, Khudaverdyan D.1, Archer C.2
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Affiliations:
- Heratci State Medical University
- Swansea University
- Issue: Vol 50, No 5 (2016)
- Pages: 300-303
- Section: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/173436
- DOI: https://doi.org/10.3103/S0095452716050145
- ID: 173436
Cite item
Abstract
Parathyroid hormone (PTH) exerts an anabolic effect that is manifested upon periodic exposure to the hormone and a catabolic effect that is observed in case of a prolonged uninterrupted exposure to high doses of the hormone. The latter effect is characteristic of a range of pathologies, such as the hyperfunction of the parathyroid glands. The analysis of the effects of periodic and continuous administration of PTH fragment 1–34 on the differentiation of bovine prechondroblasts maintained in chondrogenic and osteogenic media was the aim of the present study. Alcian blue staining of monolayers of cultured cells maintained in a chondrogenic medium revealed the stimulation of chondrogenesis (manifested as higher intensity of matrix staining) in the case of continuous PTH administration. Alizarin red staining of monolayers of cultured cells maintained in an osteogenic medium revealed more intensive mineralization of the matrix in plates with cells that were exposed to PTH in a periodical manner. The results of the histochemical study were confirmed using electrophoretic detection of osteocrin and type II collagen.
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About the authors
A. Torgomyan
Heratci State Medical University
Author for correspondence.
Email: adelinatorgomyan@yahoo.com
Armenia, ul. Koryun 2, Yerevan
S. Adamyan
Heratci State Medical University
Email: adelinatorgomyan@yahoo.com
Armenia, ul. Koryun 2, Yerevan
H. Ghambaryan
Heratci State Medical University
Email: adelinatorgomyan@yahoo.com
Armenia, ul. Koryun 2, Yerevan
H. Hasratyan
Heratci State Medical University
Email: adelinatorgomyan@yahoo.com
Armenia, ul. Koryun 2, Yerevan
D. Khudaverdyan
Heratci State Medical University
Email: adelinatorgomyan@yahoo.com
Armenia, ul. Koryun 2, Yerevan
C. Archer
Swansea University
Email: adelinatorgomyan@yahoo.com
United Kingdom, Singleton Park, Swansea, Wales, SA2 8PP