Reproductive Function of Cows with Different Genotypes for TNFα Locus and Estimation of Sperm Fertility by the DNA Fragmentation Method
- Authors: Bimenova Z.Z.1, Ussenbekov Y.S.1, Terletskiy V.P.2,3, Makashev Y.K.4, Tyshchenko V.I.3, Kassymbekova S.N.1
-
Affiliations:
- Kazakh National Agrarian University
- Pushkin Leningrad State University
- Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
- Institute of Physiology of Humans and Animals, Committee of Science, Ministry of Education and Science
- Issue: Vol 53, No 1 (2019)
- Pages: 42-48
- Section: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/174021
- DOI: https://doi.org/10.3103/S0095452719010043
- ID: 174021
Cite item
Abstract
SNP polymorphism of the TNFα gene’s promoter region at position 824 A→G in Holstein cows of TOO Bayserke-Agro is represented by the following genetic variants: AA, 22.4%; AG, 63.8%; GG, 13.8%; the frequency of alleles A and G was 0.54 and 0.46, respectively. The population under study revealed an excessive frequency of the heterozygous genotype AG, 21.49 individuals, while other genotypes showed a deficit of homozygous variants GG and AA, by 11.16 and 10.32 individuals, respectively. The traits of reproductive function were high in cows with genotype GG: the interval between calving and productive insemination was 259 days, the insemination index was 2.63, the proportion of animals inseminated after more than 91 days was minimal (47.36%) in individuals with the homozygous genotype GG. As an additional criterion for estimating the fertility of bull sperm, the DNA fragmentation method is recommended: the maximum permissible level of sperm cells with DNA fragmentation in frozen sperm is between 13.00 and 17.15%.
About the authors
Zh. Zh. Bimenova
Kazakh National Agrarian University
Author for correspondence.
Email: zhan_225@mail.ru
Kazakhstan, Almaty, 050013
Ye. S. Ussenbekov
Kazakh National Agrarian University
Author for correspondence.
Email: usen03@mail.ru
Kazakhstan, Almaty, 050013
V. P. Terletskiy
Pushkin Leningrad State University; Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the L.K. Ernst Federal Science Centerfor Animal Husbandry
Author for correspondence.
Email: valeriter@mail.ru
Russian Federation, PushkinSt. Petersburg, 196601; St. Petersburg, 196601
Ye. K. Makashev
Institute of Physiology of Humans and Animals, Committee of Science, Ministry of Education and Science
Author for correspondence.
Email: e_makashev@mail.ru
Kazakhstan, Almaty, 050060
V. I. Tyshchenko
Russian Research Institute of Farm Animal Genetics and Breeding—Branch of the L.K. Ernst Federal Science Centerfor Animal Husbandry
Author for correspondence.
Email: tinatvi@mail.ru
Russian Federation, St. Petersburg, 196601
Sh. N. Kassymbekova
Kazakh National Agrarian University
Author for correspondence.
Email: kasymbekova-s@mail.ru
Kazakhstan, Almaty, 050013