Tube Formation Potential of BMSCs and USSCs in Response to HIF-1α Overexpression under Hypoxia
- Authors: Razban V.1,2, Khajeh S.3, Alaee S.4, Mostafavi-Pour Z.3, Soleimani M.5
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Affiliations:
- Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies
- Stem Cell Technology Research Center
- Department of Biochemistry, School of Medicine
- Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies
- Department of Hematology, Faculty of Medicine
- Issue: Vol 52, No 3 (2018)
- Pages: 236-244
- Section: Article
- URL: https://journals.rcsi.science/0095-4527/article/view/173929
- DOI: https://doi.org/10.3103/S0095452718030064
- ID: 173929
Cite item
Abstract
Despite numerous studies on therapeutic effects of mesenchymal stem cells on ischemic tissue regeneration, including angiogenesis, their mechanism of action remains ambiguous. Due to the scarce of investigations based on different stem cell sources with known inherent molecular differences, present study compare tube formation of Bone marrow Mesenchymal Stem Cells and Unrestricted Somatic Stem Cells with known reported different Hox gene expression profile in response to HIF-1α overexpression under hypoxia. This might shed light on some parameters for selection of more responsive source with improved therapeutic effects. Superior in vitro tube formation on Matrigel substratum has been observed by Unrestricted Somatic Stem Cells compared to Bone marrow Mesenchymal Stem Cells which might possibly be due to the discriminating molecular properties of stem cell sources. It may help choosing the appropriate stem cell type for a given therapeutic expectations and also suggests some potential targets for future genetic modification of stem cells.
Keywords
About the authors
V. Razban
Department of Molecular Medicine, School of Advanced Medical Sciences and Technologies; Stem Cell Technology Research Center
Author for correspondence.
Email: razban_vahid@yahoo.com
Iran, Islamic Republic of, Shiraz; Shiraz
S. Khajeh
Department of Biochemistry, School of Medicine
Email: razban_vahid@yahoo.com
Iran, Islamic Republic of, Shiraz
S. Alaee
Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies
Email: razban_vahid@yahoo.com
Iran, Islamic Republic of, Shiraz
Z. Mostafavi-Pour
Department of Biochemistry, School of Medicine
Email: razban_vahid@yahoo.com
Iran, Islamic Republic of, Shiraz
M. Soleimani
Department of Hematology, Faculty of Medicine
Email: razban_vahid@yahoo.com
Iran, Islamic Republic of, Tehran