Synthesis, Spectroscopic Studies, Antibacterial Activity, and Colorimetric Evaluation of the Time-Killing Assay for Newly Synthesized Chalcones Using Resazurin
- 作者: Prasad S.1, Francis Saleshier M.1, Krishnan S.2, Bharathi P.2
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隶属关系:
- Department of Pharmaceutical Chemistry, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
- Department of Pharmaceutical Biotechnology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
- 期: 卷 52, 编号 6 (2018)
- 页面: 518-525
- 栏目: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/245300
- DOI: https://doi.org/10.1007/s11094-018-1852-z
- ID: 245300
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详细
An attempt was made to synthesize and screen chalcones active against some Gram-positive and Gram-negative bacteria by adopting modified colorimetric technique for time-killing assay. A series of chalcones were synthesized from 1-(2-bromophenyl) ethanone and various substituted aromatic aldehydes with good yield and analyzed on the basis of spectral data (FT/IR, 1H NMR, and 13C NMR). The synthesized chalcones were screened for antibacterial activity against selected Gram-positive and Gram-negative bacteria. The pharmacokinetic profiles of chalcones were established by time-kill assay performed by colorimetry using resazurin dye as an indicator which colors only the viable cells. The ethoxy (compound I-4) and para-hydroxy (I-5) derivatives were found to be more potent against Gram-positive bacteria. The colorimetric time-kill assay was found to be more convenient and less time consuming than traditional cell viable count.
作者简介
Sunnapu Prasad
Department of Pharmaceutical Chemistry, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
编辑信件的主要联系方式.
Email: prasadpharmachem@gmail.com
印度, Coimbatore, Tamil Nadu
M. Francis Saleshier
Department of Pharmaceutical Chemistry, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
Email: prasadpharmachem@gmail.com
印度, Coimbatore, Tamil Nadu
S. Krishnan
Department of Pharmaceutical Biotechnology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
Email: prasadpharmachem@gmail.com
印度, Coimbatore, Tamil Nadu
P. Bharathi
Department of Pharmaceutical Biotechnology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
Email: prasadpharmachem@gmail.com
印度, Coimbatore, Tamil Nadu