Synthesis, Spectroscopic Studies, Antibacterial Activity, and Colorimetric Evaluation of the Time-Killing Assay for Newly Synthesized Chalcones Using Resazurin
- Authors: Prasad S.1, Francis Saleshier M.1, Krishnan S.2, Bharathi P.2
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Affiliations:
- Department of Pharmaceutical Chemistry, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
- Department of Pharmaceutical Biotechnology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
- Issue: Vol 52, No 6 (2018)
- Pages: 518-525
- Section: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/245300
- DOI: https://doi.org/10.1007/s11094-018-1852-z
- ID: 245300
Cite item
Abstract
An attempt was made to synthesize and screen chalcones active against some Gram-positive and Gram-negative bacteria by adopting modified colorimetric technique for time-killing assay. A series of chalcones were synthesized from 1-(2-bromophenyl) ethanone and various substituted aromatic aldehydes with good yield and analyzed on the basis of spectral data (FT/IR, 1H NMR, and 13C NMR). The synthesized chalcones were screened for antibacterial activity against selected Gram-positive and Gram-negative bacteria. The pharmacokinetic profiles of chalcones were established by time-kill assay performed by colorimetry using resazurin dye as an indicator which colors only the viable cells. The ethoxy (compound I-4) and para-hydroxy (I-5) derivatives were found to be more potent against Gram-positive bacteria. The colorimetric time-kill assay was found to be more convenient and less time consuming than traditional cell viable count.
About the authors
Sunnapu Prasad
Department of Pharmaceutical Chemistry, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
Author for correspondence.
Email: prasadpharmachem@gmail.com
India, Coimbatore, Tamil Nadu
M. Francis Saleshier
Department of Pharmaceutical Chemistry, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
Email: prasadpharmachem@gmail.com
India, Coimbatore, Tamil Nadu
S. Krishnan
Department of Pharmaceutical Biotechnology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
Email: prasadpharmachem@gmail.com
India, Coimbatore, Tamil Nadu
P. Bharathi
Department of Pharmaceutical Biotechnology, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences
Email: prasadpharmachem@gmail.com
India, Coimbatore, Tamil Nadu