Email this article Microcolumn HPLC-UV Analysis of Glycyrrhiza Uralensis and Licorice Preparations
- Authors: Vennos C.1, Olennikov D.N.2, Zilfikarov I.N.3
-
Affiliations:
- Regulatory and Medical Scientific Affairs
- Institute of General and Experimental Biology Siberian Branch, Russian Academy of Sciences
- Scientific Center of Biomedical Technologies, All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR)
- Issue: Vol 52, No 12 (2019)
- Pages: 986-991
- Section: Article
- URL: https://journals.rcsi.science/0091-150X/article/view/245570
- DOI: https://doi.org/10.1007/s11094-019-01938-9
- ID: 245570
Cite item
Open Access
Access granted
Subscription Access
Abstract
A microcolumn HPLC method with UV detection (MC-HPLC-UV) using ProntoSIL-120-5-C18 sorbent (1 × 60 mm, 1 μm) and gradient elution by LiClO4(4.1 M)/HClO4(0.1 M)–MeCN was developed for quantitative analysis of seven compounds (liquiritin-2″-O-glucoside, liquiritin, isoliquiritin-2″-O-apioside, isoliquiritin, licorice-saponin G2, yunganoside E2, glycyrrhizic acid) in Glycyrrhiza uralensis Fisch. roots. Validation of the procedure showed that the metrological parameters were satisfactory. The procedure was used for research on G. uralensis roots growing in Siberia and quantitative analysis of glycyrrhizic acid in 28 commercial licorice preparations.
About the authors
Cecile Vennos
Regulatory and Medical Scientific Affairs
Email: olennikovdn@mail.ru
Switzerland, Padma AG, Hinwil
D. N. Olennikov
Institute of General and Experimental Biology Siberian Branch, Russian Academy of Sciences
Author for correspondence.
Email: olennikovdn@mail.ru
Russian Federation, Buryat Republic, Ulan-Ude, 670047
I. N. Zilfikarov
Scientific Center of Biomedical Technologies, All-Russia Research Institute of Medicinal and Aromatic Plants (VILAR)
Email: olennikovdn@mail.ru
Russian Federation, Moscow, 117216
