Microcolumn HPLC-UV Analysis of Glycyrrhiza Uralensis and Licorice Preparations

详细

A microcolumn HPLC method with UV detection (MC-HPLC-UV) using ProntoSIL-120-5-C18 sorbent (1 × 60 mm, 1 μm) and gradient elution by LiClO₄(4.1 M)/HClO₄(0.1 M)–MeCN was developed for quantitative analysis of seven compounds (liquiritin-2″-O-glucoside, liquiritin, isoliquiritin-2″-O-apioside, isoliquiritin, licorice-saponin G2, yunganoside E2, glycyrrhizic acid) in Glycyrrhiza uralensis Fisch. roots. Validation of the procedure showed that the metrological parameters were satisfactory. The procedure was used for research on G. uralensis roots growing in Siberia and quantitative analysis of glycyrrhizic acid in 28 commercial licorice preparations.