Phytochemical Investigation, Antioxidant and Antimicrobial Assays of Algerian Plant Calamintha baborensis Batt.

Abstract

The aim of this work was to evaluate the antioxidant capacity of some increasing polarity soluble fractions of hydroalcoholic extract of the leaves of Algerian Lamiaceae Calamintha baborensis Batt., including hexanoic (Hex), chloroformic (Chlor), ethyl acetate (EtOAc) and n-butanolic (n-BuOH) and four subfractions (A, B, C, D) resulting from fractionation on a polyamide column. The EtOAc and n-BuOH extracts of the plant gave the highest values of antioxidant power by different methods: ABTS, percentage inhibition of 68.9/81.7%; DPPH, 27.6/80.99%; ORAC/FRAP, 37.28/28.47 and 21.73/19.52 μM/mL, respectively; IC50 values, 23 and 53.5 ppm. In addition, they were evaluated for their total phenolic content. The antibacterial activity of extracts showed good results with hexanoic and chloroformic fractions against E. coli (19 mm and 19.2 mm diameter of inhibition zone and MIC values about 43 and 43.4 μg/mL, respectively.) Apolar fractions from the leaves of C. Baborensis Batt. were analyzed by gas chromatography–mass spectrometry (GC–MS). The main components of the essential oil of C baborensis Batt. are eugenol (27.04%) and 3-methoxy acetophenone (26.4%).