Quantitative Determination of Mycophenolic Acid in Human Blood Plasma by High-Performance Liquid Chromatography with Tandem Mass-Spectrometric Detection

A. L. Khokhlov; Yu. A. Dzhurko; L. N. Shitov; V. Kubeš; M. Ryska; I. I Yaichkov; A. M. Shitova; V. N. Shabrov; A. E. Miroshnikov

doi:10.1007/s11094-017-1643-y

Quantitative Determination of Mycophenolic Acid in Human Blood Plasma by High-Performance Liquid Chromatography with Tandem Mass-Spectrometric Detection

Authors: Khokhlov A.L.¹^,2, Dzhurko Y.A.², Shitov L.N.¹^,2, Kubeš V.², Ryska M.², Yaichkov I.I¹^,2, Shitova A.M.², Shabrov V.N.², Miroshnikov A.E.¹
Affiliations:
1. Yaroslavl State Medical University, Ministry of Public Health of the Russian Federation
2. Quinta-Analytica Yaroslavl Co.
Issue: Vol 51, No 6 (2017)
Pages: 504-507
Section: Article
URL: https://journals.rcsi.science/0091-150X/article/view/244678
DOI: https://doi.org/10.1007/s11094-017-1643-y
ID: 244678

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Abstract
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Abstract

A new rapid and accurate method for determining the mycophenolic acid concentration in human blood plasma using HPLC with tandem mass-spectrometric detection (HPLC-MS/MS) and deproteinization for sample preparation was developed. The method was validated according to the Guideline for Validation of Bioanalytical Methods of EMEA and Guidelines for Examination of Drugs. This method was used to study the pharmacokinetics of mycophenolate sodium.

Keywords

mycophenolic acid, deproteinization, HPLC-MS/MS, validation, pharmacokinetics

About the authors

A. L. Khokhlov

Yaroslavl State Medical University, Ministry of Public Health of the Russian Federation; Quinta-Analytica Yaroslavl Co.

Email: chem@folium.ru
Russian Federation, 5 Revolyutsionnaya St, Yaroslavl, 150000; 52 Leningradskii Prosp, Yaroslavl, 150045