Acute Toxicity of Substituted Hydroquinones Evaluated by a Modified Probit Analysis Method

Abstract

The LD₅₀ values of methyl-, bromo-, and chlorohydroquinones were evaluated. Probit analysis calculations by the Bliss—Prozorovsky least-squares method were optimized using preliminary results of the OECD TG 425 Up-and-Down Procedure. The proposed modification allowed economic expenditures to be diminished by reducing the numbers of injected doses and experimental animals and could be applied to testing of substances by other drug administration modes. Correlation functions between the toxicity values and dissociation constants were obtained and allowed the LD₅₀ values of the hydroquinone derivatives to be evaluated.