Assessment of Total Polyphenol Compounds in the Complex Phytopreparations Angionorm and Prostanorm Using the Folin-Ciocalteu Method

Abstract

Studies reported here evaluated the applicability of the Folin-Ciocalteu method for assessing total phenolic compounds in the complex phytopreparation Angionorm, which is based on dry extract of a mixture of medicinal herb substances (MHS), i.e., seeds (fruits) of the common horse chestnut, licorice roots, hawthorn fruits, and dog rose fruits at a ratio of 30:15:20:35, and preparation Prostanorm, consisting of a 50% aqueous-alcoholic extract of a mixture of four MHS, i.e., St. John’s wort herb, Canadian goldenrod herb, licorice root, Echinacea rhizomes and roots in equal proportions by weight, and a preparation consisting of a dry extract based on this mixture. The method was adapted to analysis of total polyphenol content in medicinal formulations using these preparations. The method was validated in terms of specificity, correctness, linearity, and similarity, and satisfied the suitability criteria. Total polyphenol contexts were assayed as gallic acid equivalents. A decolorization method using the cationic radical ABTS was used to determine the antiradical activity of Angionorm and Prostanorm in trolox equivalent antioxidant capacity (TEAC) units and in terms of the IC₅₀ (the concentration inhibiting activity by 50%). A correlation was found between the total content of phenolic compounds and antiradical activity.