Diversity of transcripts of toll-like receptors in hemocytes of Planorbarius corneus mollusсs (Gastropoda, Pulmonata) uninfected and infected with Bilharziella polonica trematodes

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Transcriptome of hemocytes from uninfected Planorbarius corneus snails and snails naturally infected with Bilharziella polonica (Schistosomatidae) was obtained and analyzed. Transcripts encoding all groups of immune factors previously described for other gastropods were found. Pathogen recognition molecules are the most diverse group of transcripts encoding immunity factors. Toll-like receptors of 11 types were identified. No differences in the set of toll-like receptors between infected and uninfected mollusсs were found. The data obtained expand our knowledge on immune reactions of snails to trematode invasion and make it possible to consider Planorbarius corneus as a new model for studying molluscs immune reactions.

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作者简介

А. Bobrovskaya

Российский государственный педагогический университет им. А.И. Герцена

Email: elenne@mail.ru

кафедра зоологии и генетики, лаборатория экспериментальной зоологии

俄罗斯联邦, наб. р. Мойки, д. 48, Санкт-Петербург, 191186

I. Orlov

Российский государственный педагогический университет им. А.И. Герцена

Email: elenne@mail.ru

кафедра зоологии и генетики, лаборатория экспериментальной зоологии

俄罗斯联邦, наб. р. Мойки, д. 48, Санкт-Петербург, 191186

E. Prokhorova

Российский государственный педагогический университет им. А.И. Герцена

编辑信件的主要联系方式.
Email: elenne@mail.ru

кафедра зоологии и генетики, лаборатория экспериментальной зоологии

俄罗斯联邦, наб. р. Мойки, д. 48, Санкт-Петербург, 191186

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2. Figure 1. The number of pathogen-recognizing receptors (PRR) and adhesion molecules domains in hemocytes of the mollusk Planorbarius corneus according to BlastP (databases NCBI NR, e-value<1e-5).

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3. Figure 2. Phylogenetic reconstruction based on amino-acid sequences of TLR TIR-domain in the mollusc Planornarius corneus (PсTLR), performed by the neighbor joining method (NJ). A tree obtained by the maximum likelihood estimation method (ML) demonstrated similar topology. Bootstrep supports of 1000 replicas for NJ/ML are designated. Numbers of the used sequences in GenBank are indicated.

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4. Figure 3. Variants of the predicted TLR domain structure in hemocytes of the molluscs Planorbarius corneus (A) and relative representation of TLR transcripts in infected and uninfected molluscs (Б), expressed in TPM. Horizontal rep stripes – a signal peptide, horizontal pink stripes – the low complexity area; vertical blue stripes – transmembrane area; LRR – rich in leucine repetition; LRR_TYP – rich in leucine repetition ща the typical subfamily domain; LRR_CT – rich in leucine С-terminal repetition domain; LRR NT– rich in leucine N-terminal repetition domain; TIR – TIR-domain; Н – uninfected mollusсs; З – molluscs infected by Bilharziella polonica.

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