Construction of Vectors for the Genome Editing of Saccharomyces Yeast Using CRISPR-Cas9 System
- 作者: Matveenko A.1, Mikhailichenko A.1, Zhouravleva G.1,2
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隶属关系:
- St Petersburg State University
- Laboratory of Amyloid Biology SPbU
- 期: 卷 93, 编号 2 (2024)
- 页面: 139-144
- 栏目: КРАТКИЕ СООБЩЕНИЯ
- URL: https://journals.rcsi.science/0026-3656/article/view/262489
- DOI: https://doi.org/10.31857/S0026365624020073
- ID: 262489
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详细
New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.
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作者简介
A. Matveenko
St Petersburg State University
Email: g.zhuravleva@spbu.ru
俄罗斯联邦, St Petersburg, 199034
A. Mikhailichenko
St Petersburg State University
Email: g.zhuravleva@spbu.ru
俄罗斯联邦, St Petersburg, 199034
G. Zhouravleva
St Petersburg State University; Laboratory of Amyloid Biology SPbU
编辑信件的主要联系方式.
Email: g.zhuravleva@spbu.ru
俄罗斯联邦, St Petersburg, 199034; St Petersburg, 199034
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