Molecular Genetic and Functional Analysis of the Rap-Phr Signal System of the Plasmid pBS72 of Bacillus subtilis Isolates

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Abstract—

The functional characteristics of the Rap-Phr quorum sensing signal system of the plasmid pBS72 were investigated. Phylogenetic relationship was revealed between Rap phosphatase coded by the plasmid pBS72 and the homologous polypeptides RapP and RapI determined by the plasmid pBS32 (68.4% identity) and the ICEBs1 conjugative transposon (36.9% identity). Similar to the phylogenetically related phosphatases, the studied Rap protein had a negative effect on sporulation. Unlike the known signal systems, Rap-Phr proteins were found to affect the viability of plasmid-bearing donor bacteria in the course of conjugative transfer of the plasmid pBS72 in the isogenic system. Impaired rap-phr genes resulted in a 10-fold decrease in the number of viable donor cells with the mutant plasmid after 3 and 4 h, and in a 100-fold decrease after 24 h. The number of formed transconjugants remained almost the same. Our results provide the basis for investigation of the mechanisms responsible for the effect of extrachromosomal genetic elements on the donor bacteria, providing for the propagation of the pBS72-like plasmids in natural environments.

作者简介

A. Gurinovich

Department of Microbiology, Belarusian State University

编辑信件的主要联系方式.
Email: m_titok@bsu.by
Belarus, 220030, Minsk

M. Titok

Department of Microbiology, Belarusian State University

编辑信件的主要联系方式.
Email: nastia.gurinovich96@gmail.com
Belarus, 220030, Minsk

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版权所有 © А.С. Гуринович, М.А. Титок, 2023

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