Effect of the AZOBR_p60123 Plasmid Gene, Encoding the Wzt Protein, on Lipopolysaccharide Synthesis and Biofilm Formation in the Bacterium Azospirillum baldaniorum Sp245
- Авторлар: Petrova L.1, Evstigneeva S.1, Filip’echeva Y.1, Volokhina I.1, Burygin G.1, Matora .1, Shelud’ko A.1
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Мекемелер:
- Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center, Russian Academy of Sciences
- Шығарылым: Том 92, № 3 (2023)
- Беттер: 289-299
- Бөлім: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
- URL: https://journals.rcsi.science/0026-3656/article/view/138217
- DOI: https://doi.org/10.31857/S002636562260081X
- EDN: https://elibrary.ru/FWBWKP
- ID: 138217
Дәйексөз келтіру
Аннотация
Abstract—
Inactivation of one of the genes (CDS AZOBR_p60123) of the ABC transporter ATP-binding protein Wzt in the p60123::aphAI mutant of Azospirillum baldaniorum Sp245 and the introduction of an additional copy of this gene into the parent or mutant strain affected the chain-length heterogeneity of O polysaccharides (OPSs) and lipopolysaccharides (LPSs), the OPS and LPS immunochemical characteristics, and the strain properties related to polysaccharide production (biofilm construction). Biofilms of the p60123::aphAI mutant Sp245.4-1-1 accumulated two times less biomass than those of Sp245. Introduction of pRK415-p60123 into the cells of Sp245.4-1-1 or Sp245 resulted in a respective increase or decrease in the ability of the resulting derivative strains to accumulate biofilm biomass, as compared with the original strains.
Негізгі сөздер
Авторлар туралы
L. Petrova
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center,Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: petrova_lp@mail.ru
Russia, 410049, Saratov
S. Evstigneeva
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center,Russian Academy of Sciences
Email: shel71@yandex.ru
Russia, 410049, Saratov
Y. Filip’echeva
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center,Russian Academy of Sciences
Email: shel71@yandex.ru
Russia, 410049, Saratov
I. Volokhina
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center,Russian Academy of Sciences
Email: shel71@yandex.ru
Russia, 410049, Saratov
G. Burygin
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center,Russian Academy of Sciences
Email: shel71@yandex.ru
Russia, 410049, Saratov
L. Matora
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center,Russian Academy of Sciences
Email: shel71@yandex.ru
Russia, 410049, Saratov
A. Shelud’ko
Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Center,Russian Academy of Sciences
Хат алмасуға жауапты Автор.
Email: shel71@yandex.ru
Russia, 410049, Saratov
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