Detection of Microcystin-Producing Cyanobacteria Microcystis, Planktothrix, and Dolichospermum Using Multiprimer Amplification of the mcy Genes

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Аннотация

Abstract—A multiprimer PCR-based technique for the joint molecular detection of microcystin-producing planktonic cyanobacteria of the genera Microcystis, Planktothrix and Dolichospermum was developed. To amplify the mcy genes of microcystin biosynthesis, three pairs of genus-specific primers were selected, while the fourth pair was used to amplify the inter-gene spacer (IGS) cpcBA acting as an inner positive control for the presence of cyanobacterial DNA in the analyzed samples. Four PCR products of predicted size were successfully amplified using a mixture of DNA templates isolated from microсystin-producing strains of M. aeruginosa, P. agardhii, and field colonies of D. lemmermannii colonies retrieved from the environment, as well as аn environmental DNA template from lake plankton. The proposed multiprimer PCR-based approach may be used to design convenient test systems for monitoring aquatic ecosystems to prevent and/or assess the risk of accumulation of harmful cyanobacterial toxins.

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Авторлар туралы

S. Sidelev

Yaroslavl State University

Хат алмасуға жауапты Автор.
Email: Sidelev@mail.ru
Russia, 150057, Yaroslavl

Әдебиет тізімі

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